| RRC ID |
5549
|
| 著者 |
Araki K, Takeda N, Yoshiki A, Obata Y, Nakagata N, Shiroishi T, Moriwaki K, Yamamura K.
|
| タイトル |
Establishment of germline-competent embryonic stem cell lines from the MSM/Ms strain.
|
| ジャーナル |
Mamm Genome
|
| Abstract |
MSM/Ms is an inbred mouse strain established from the Japanese wild mouse, Mus musculus molossinus, which has been phylogenetically distinct from common laboratory mouse strains for about 1 million years. The nucleotide substitution rate between MSM/Ms and C57BL/6 is estimated to be 0.96%. MSM/Ms mice display unique characteristics not observed in the commonly used laboratory strains, including an extremely low incidence of tumor development, high locomotor activity, and resistance to high-fat-diet-induced diabetes. Thus, functional genomic analyses using MSM/Ms should provide a powerful tool for the identification of novel phenotypes and gene functions. We report here the derivation of germline-competent embryonic stem (ES) cell lines from MSM/Ms blastocysts, allowing genetic manipulation of the M. m. molossinus genome. Fifteen blastocysts were cultured in ES cell medium and three ES lines, Mol/MSM-1, -2, and -3, were established. They were tested for germline competency by aggregation with ICR morulae and germline chimeras were obtained from all three lines. We also injected Mol/MSM-1 ES cells into blastocysts of ICR or C57BL/6 x BDF1 mice and found that blastocyst injection resulted in a higher production rate of chimeric mice than did aggregation. Furthermore, Mol/MSM-1 subclones electroporated with a gene trap vector were also highly efficient at producing germline chimeras using C57BL/6 x BDF1 blastocyst injection. This Mol/MSM-1 ES line should provide an excellent new tool allowing the genetic manipulation of the MSM/Ms genome.
|
| 巻・号 |
20(1)
|
| ページ |
14-20
|
| 公開日 |
2009-1-1
|
| DOI |
10.1007/s00335-008-9160-7
|
| PMID |
19082856
|
| MeSH |
Animals
Animals, Wild / embryology
Cell Differentiation / physiology
Cell Line*
Chimera / embryology
Crosses, Genetic
Electroporation
Embryo Culture Techniques
Embryonic Stem Cells / cytology*
Embryonic Stem Cells / physiology*
Female
Germ Cells / physiology*
Male
Mice
Mice, Inbred C57BL
Mice, Inbred ICR
Mice, Inbred Strains
|
| IF |
2.287
|
| 引用数 |
13
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
GENETICS & HEREDITY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 実験動物マウス |
RBRC00209 |
