| RRC ID |
55507
|
| 著者 |
Kawashima T, Ikari N, Kouchi T, Kowatari Y, Kubota Y, Shimojo N, Tsuji NM.
|
| タイトル |
The molecular mechanism for activating IgA production by Pediococcus acidilactici K15 and the clinical impact in a randomized trial.
|
| ジャーナル |
Sci Rep
|
| Abstract |
IgA secretion at mucosal sites is important for host defence against pathogens as well as maintaining the symbiosis with microorganisms present in the small intestine that affect IgA production. In the present study, we tested the ability of 5 strains of lactic acid bacteria stimulating IgA production, being Pediococcus acidilactici K15 selected as the most effective on inducing this protective immunoglobulin. We found that this response was mainly induced via IL-10, as efficiently as IL-6, secreted by K15-stimulated dendritic cells. Furthermore, bacterial RNA was largely responsible for the induction of these cytokines; double-stranded RNA was a major causative molecule for IL-6 production whereas single-stranded RNA was critical factor for IL-10 production. In a randomized, double-blind, placebo-controlled clinical trial, ingestion of K15 significantly increased the secretory IgA (sIgA) concentration in saliva compared with the basal level observed before this intervention. These results indicate that functional lactic acid bacteria induce IL-6 and IL-10 production by dendritic cells, which contribute to upregulating the sIgA concentration at mucosal sites in humans.
|
| 巻・号 |
8(1)
|
| ページ |
5065
|
| 公開日 |
2018-3-22
|
| DOI |
10.1038/s41598-018-23404-4
|
| PII |
10.1038/s41598-018-23404-4
|
| PMID |
29567956
|
| PMC |
PMC5864838
|
| MeSH |
Adult
Animals
Dendritic Cells / metabolism
Dendritic Cells / microbiology
Female
Gastric Mucosa / metabolism
Gastric Mucosa / microbiology
Humans
Immunoglobulin A, Secretory / biosynthesis*
Immunoglobulin A, Secretory / metabolism
Interleukin-10 / biosynthesis*
Interleukin-10 / genetics
Interleukin-6 / biosynthesis*
Interleukin-6 / genetics
Intestine, Small / metabolism*
Intestine, Small / microbiology
Lactobacillales / immunology
Lactobacillales / metabolism
Male
Middle Aged
Pediococcus acidilactici / immunology
Pediococcus acidilactici / metabolism*
RNA, Bacterial / genetics
RNA, Bacterial / metabolism
RNA, Double-Stranded / genetics
RNA, Double-Stranded / metabolism
Saliva / metabolism
Saliva / microbiology
|
| IF |
4.011
|
| 引用数 |
13
|
| リソース情報 |
| 一般微生物 |
JCM 1149
JCM 1558
JCM 1002 |
