RRC ID 55510
著者 Tanaka K, Satoh T, Kitahara J, Uno S, Nomura I, Kano Y, Suzuki T, Niimura Y, Kawasaki S.
タイトル O2-inducible H2O2-forming NADPH oxidase is responsible for the hyper O2 sensitivity of Bifidobacterium longum subsp. infantis.
ジャーナル Sci Rep
Abstract Bifidobacteria are beneficial anaerobes, and their O2 sensitivity levels differ among species as a function of unknown molecular mechanisms. Bifidobacterium longum subspecies infantis (B. infantis), a predominant colonizer of the gastrointestinal tract of infants, showed a hyper O2-sensitive growth profile with accompanying a production of H2O2. In this study, we characterized an NADPH oxidase as a key enzyme responsible for this microbe's hyper O2 sensitivity. A dominant active elution peak of H2O2-forming NADPH oxidase activity was detected in the first step of column chromatography, and the purified NADPH oxidase (NPOX) was identified as a homolog of nitroreductase family proteins. The introduction of the gene encoding B. infantis NPOX (npoxA) into O2-tolerant Bifidobacterium minimum made the strain O2 sensitive and allowed it to produce H2O2. Knockout of the npoxA gene in B. infantis decreased the production of H2O2 and mitigated its B. infantis hyper O2 sensitivity. A transcript of B. infantis npoxA is induced by O2, suggesting that the aerobic production of toxic H2O2 is functionally conserved in B. infantis.
巻・号 8(1)
ページ 10750
公開日 2018-7-16
DOI 10.1038/s41598-018-29030-4
PII 10.1038/s41598-018-29030-4
PMID 30013208
PMC PMC6048055
MeSH Bacteria, Anaerobic / genetics Bacteria, Anaerobic / metabolism Bacterial Proteins / genetics Bacterial Proteins / metabolism* Bifidobacterium longum subspecies infantis / enzymology* Bifidobacterium longum subspecies infantis / genetics DNA, Bacterial / genetics Gene Knockout Techniques Hydrogen Peroxide / metabolism* NADPH Oxidases / genetics NADPH Oxidases / metabolism* Oxidative Stress Oxygen / toxicity*
IF 4.011
引用数 2
リソース情報
一般微生物 JCM 1222