論文 - 詳細
| RRC ID | 55513 |
|---|---|
| 著者 | Mavrich TN, Casey E, Oliveira J, Bottacini F, James K, Franz CMAP, Lugli GA, Neve H, Ventura M, Hatfull GF, Mahony J, van Sinderen D. |
| タイトル | Characterization and induction of prophages in human gut-associated Bifidobacterium hosts. |
| ジャーナル | Sci Rep |
| Abstract |
In the current report, we describe the identification of three genetically distinct groups of prophages integrated into three different chromosomal sites of human gut-associated Bifidobacterium breve and Bifidobacterium longum strains. These bifidobacterial prophages are distantly related to temperate actinobacteriophages of several hosts. Some prophages, integrated within the dnaJ2 gene, are competent for induction, excision, replication, assembly and lysis, suggesting that they are fully functional and can generate infectious particles, even though permissive hosts have not yet been identified. Interestingly, several of these phages harbor a putative phase variation shufflon (the Rin system) that generates variation of the tail-associated receptor binding protein (RBP). Unlike the analogous coliphage-associated shufflon Min, or simpler Cin and Gin inversion systems, Rin is predicted to use a tyrosine recombinase to promote inversion, the first reported phage-encoded tyrosine-family DNA invertase. The identification of bifidobacterial prophages with RBP diversification systems that are competent for assembly and lysis, yet fail to propagate lytically under laboratory conditions, suggests dynamic evolution of bifidobacteria and their phages in the human gut. |
| 巻・号 | 8(1) |
| ページ | 12772 |
| 公開日 | 2018-8-24 |
| DOI | 10.1038/s41598-018-31181-3 |
| PII | 10.1038/s41598-018-31181-3 |
| PMID | 30143740 |
| PMC | PMC6109161 |
| MeSH | Attachment Sites, Microbiological / genetics Base Sequence Bifidobacterium / drug effects Bifidobacterium / virology* Biological Evolution Gastrointestinal Microbiome* / drug effects Genome, Viral Host Specificity / drug effects Host Specificity / genetics Humans Mitomycin / pharmacology Prophages / drug effects Prophages / genetics Prophages / physiology* Prophages / ultrastructure Virion / drug effects Virus Replication / drug effects |
| IF | 4.011 |
| 引用数 | 6 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 48 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 7017 |