| RRC ID |
55526
|
| 著者 |
Sorokin DY, Khijniak TV, Kostrikina NA, Elcheninov AG, Toshchakov SV, Bale NJ, Damsté JSS, Kublanov IV.
|
| タイトル |
Natronobiforma cellulositropha gen. nov., sp. nov., a novel haloalkaliphilic member of the family Natrialbaceae (class Halobacteria) from hypersaline alkaline lakes.
|
| ジャーナル |
Syst Appl Microbiol
|
| Abstract |
Six strains of extremely halophilic and alkaliphilic euryarchaea were enriched and isolated in pure culture from surface brines and sediments of hypersaline alkaline lakes in various geographical locations with various forms of insoluble cellulose as growth substrate. The cells are mostly flat motile rods with a thin monolayer cell wall while growing on cellobiose. In contrast, the cells growing with cellulose are mostly nonmotile cocci covered with a thick external EPS layer. The isolates, designated AArcel, are obligate aerobic heterotrophs with a narrow substrate spectrum. All strains can use insoluble celluloses, cellobiose, a few soluble glucans and xylan as their carbon and energy source. They are extreme halophiles, growing within the range from 2.5 to 4.8M total Na+ (optimum at 4M) and obligate alkaliphiles, with the pH range for growth from 7.5 to 9.9 (optimum at 8.5-9). The core archaeal lipids of strain AArcel5T were dominated by C20-C20 dialkyl glycerol ether (DGE) (i.e. archaeol) and C20-C25 DGE in nearly equal proportion. The 16S rRNA gene analysis indicated that all six isolates belong to a single genomic species mostly related to the genera Saliphagus-Natribaculum-Halovarius. Taking together a substantial phenotypic difference of the new isolates from the closest relatives and the phylogenetic distance, it is concluded that the AArcel group represents a novel genus-level branch within the family Natrialbaceae for which the name Natronobiforma cellulositropha gen. nov., sp. nov. is proposed with AArcel5T as the type strain (JCM 31939T=UNIQEM U972T).
|
| 巻・号 |
41(4)
|
| ページ |
355-362
|
| 公開日 |
2018-7-1
|
| DOI |
10.1016/j.syapm.2018.04.002
|
| PII |
S0723-2020(18)30095-X
|
| PMID |
29752017
|
| PMC |
PMC6052348
|
| MeSH |
Cellobiose / metabolism
DNA, Bacterial / genetics
Fatty Acids / analysis
Halobacteriales* / classification
Halobacteriales* / genetics
Halobacteriales* / isolation & purification
Lakes / microbiology*
Phylogeny
RNA, Ribosomal, 16S / genetics
Salinity*
Sequence Analysis, DNA
Sodium Chloride / analysis*
|
| IF |
2.808
|
| 引用数 |
9
|
| リソース情報 |
| 一般微生物 |
JCM 31939 |
