| RRC ID |
5554
|
| 著者 |
Teramoto K, Kitagawa W, Sato H, Torimura M, Tamura T, Tao H.
|
| タイトル |
Phylogenetic analysis of Rhodococcus erythropolis based on the variation of ribosomal proteins as observed by matrix-assisted laser desorption ionization-mass spectrometry without using genome information.
|
| ジャーナル |
J Biosci Bioeng
|
| Abstract |
Rhodococcus erythropolis strains characterized as antibiotic producers can be classified into three groups according to their antibiotic spectrum and growth compatibility. Due to their high genotypic similarity, the taxonomic relationship of these strains has not been elucidated. In this study, ribosomal protein profiling using matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) was employed to classify twenty-one strains of R. erythropolis (15 antibiotic producers and 6 non-antibiotic producers). In the first step in this method, a total of 30 intense peaks observed for purified ribosomal subunit proteins of the type strain (R. erythropolis JCM 3201(T)) were selected as the reference peaks. The mass spectra observed for the cell lysates of each sample strain were then checked as to whether peaks were observed at the same masses of the reference peaks. The results of peak matching were processed by cluster analysis, generating a dendrogram. Four major clusters of the R. erythropolis strains corresponded to three antibiotic groups and the non-antibiotic group. Furthermore, the topology of the dendrogram was highly comparable with the phylogenetic tree based on DNA gyrase subunit B gene (gyrB) sequencing. These results indicate that our proposed ribosomal protein profiling method using MALDI-MS is a potentially reliable and sufficiently high-throughput technique for the taxonomic analysis of closely related bacterial strains without using DNA sequence information.
|
| 巻・号 |
108(4)
|
| ページ |
348-53
|
| 公開日 |
2009-10-1
|
| DOI |
10.1016/j.jbiosc.2009.04.010
|
| PII |
S1389-1723(09)00202-3
|
| PMID |
19716527
|
| MeSH |
Anti-Bacterial Agents / biosynthesis
Bacterial Proteins / genetics*
Cluster Analysis
DNA Gyrase / genetics
Gene Expression Profiling / methods
Genome, Bacterial
Rhodococcus / classification*
Rhodococcus / genetics*
Ribosomal Proteins / genetics*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
|
| IF |
2.366
|
| 引用数 |
21
|
|
WOS 分野
|
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM3201 |
