| RRC ID |
556
|
| 著者 |
Kojima KK, Matsumoto T, Fujiwara H.
|
| タイトル |
Eukaryotic translational coupling in UAAUG stop-start codons for the bicistronic RNA translation of the non-long terminal repeat retrotransposon SART1.
|
| ジャーナル |
Mol Cell Biol
|
| Abstract |
Most eukaryotic cellular mRNAs are monocistronic; however, many retroviruses and long terminal repeat (LTR) retrotransposons encode multiple proteins on a single RNA transcript using ribosomal frameshifting. Non-long terminal repeat (non-LTR) retrotransposons are considered the ancestor of LTR retrotransposons and retroviruses, but their translational mechanism of bicistronic RNA remains unknown. We used a baculovirus expression system to produce a large amount of the bicistronic RNA of SART1, a non-LTR retrotransposon of the silkworm, and were able to detect the second open reading frame protein (ORF2) by Western blotting. The ORF2 protein was translated as an independent protein, not as an ORF1-ORF2 fusion protein. We revealed by mutagenesis that the UAAUG overlapping stop-start codon and the downstream RNA secondary structure are necessary for efficient ORF2 translation. Increasing the distance between the ORF1 stop codon and the ORF2 start codon decreased translation efficiency. These results are different from the eukaryotic translation reinitiation mechanism represented by the yeast GCN4 gene, in which the probability of reinitiation increases as the distance between the two ORFs increases. The translational mechanism of SART1 ORF2 is analogous to translational coupling observed in prokaryotes and viruses. Our results indicate that translational coupling is a general mechanism for bicistronic RNA translation.
|
| 巻・号 |
25(17)
|
| ページ |
7675-86
|
| 公開日 |
2005-9-1
|
| DOI |
10.1128/MCB.25.17.7675-7686.2005
|
| PII |
25/17/7675
|
| PMID |
16107714
|
| PMC |
PMC1190309
|
| MeSH |
Amino Acid Sequence
Antigens, Neoplasm / chemistry
Antigens, Neoplasm / genetics*
Base Sequence
Codon, Initiator / genetics*
Codon, Terminator / genetics*
Frameshifting, Ribosomal
Molecular Sequence Data
Nucleic Acid Conformation
Open Reading Frames / genetics
Protein Biosynthesis / genetics*
RNA, Fungal / chemistry
RNA, Fungal / genetics
RNA, Fungal / metabolism
Retroelements / genetics*
Ribonucleoproteins, Small Nuclear / chemistry
Ribonucleoproteins, Small Nuclear / genetics*
Saccharomyces cerevisiae / genetics*
|
| IF |
3.611
|
| 引用数 |
30
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| カイコ |
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