RRC ID 55609
Author Nishida I, Yokomi K, Hosono K, Hayashi K, Matsuo Y, Kaino T, Kawamukai M.
Title CoQ10 production in Schizosaccharomyces pombe is increased by reduction of glucose levels or deletion of pka1.
Journal Appl Microbiol Biotechnol
Abstract Coenzyme Q (CoQ) is an essential component of the electron transport system that produces ATP in nearly all living cells. CoQ10 is a popular commercial food supplement around the world, and demand for efficient production of this molecule has increased in recent years. In this study, we explored CoQ10 production in the fission yeast Schizosaccharomyces pombe. We found that CoQ10 level was higher in stationary phase than in log phase, and that it increased when the cells were grown in a low concentration of glucose, in maltose, or in glycerol/ethanol medium. Because glucose signaling is mediated by cAMP, we evaluated the involvement of this pathway in CoQ biosynthesis. Loss of Pka1, the catalytic subunit of cAMP-dependent protein kinase, increased production of CoQ10, whereas loss of the regulatory subunit Cgs1 decreased production. Manipulation of other components of the cAMP-signaling pathway affected CoQ10 production in a consistent manner. We also found that glycerol metabolism was controlled by the cAMP/PKA pathway. CoQ10 production by the S. pombe ∆pka1 reached 0.98 mg/g dry cell weight in medium containing a non-fermentable carbon source [2% glycerol (w/v) and 1% ethanol (w/v) supplemented with 0.5% casamino acids (w/v)], twofold higher than the production in wild-type cells under normal growth conditions. These findings demonstrate that carbon source, growth phase, and the cAMP-signaling pathway are important factors in CoQ10 production in S. pombe.
Volume 103(12)
Pages 4899-4915
Published 2019-6-1
DOI 10.1007/s00253-019-09843-7
PII 10.1007/s00253-019-09843-7
PMID 31030285
MeSH Catalytic Domain Culture Media / chemistry Cyclic AMP-Dependent Protein Kinases / genetics* Cyclic AMP-Dependent Protein Kinases / metabolism Fungal Proteins / genetics* Fungal Proteins / metabolism Gene Deletion Glucose / chemistry* Maltose / chemistry Schizosaccharomyces / metabolism* Signal Transduction Ubiquinone / analogs & derivatives* Ubiquinone / biosynthesis Ubiquinone / metabolism
IF 3.67
Times Cited 0
Yeast FY33782, FY33783