RRC ID 55611
著者 Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.
タイトル Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.
ジャーナル PLoS Genet
Abstract Endocycle is a commonly observed cell cycle variant through which cells undergo repeated rounds of genome DNA replication without mitosis. Endocycling cells arise from mitotic cells through a switch of the cell cycle mode, called the mitotic-to-endocycle switch (MES), to initiate cell growth and terminal differentiation. However, the underlying regulatory mechanisms of MES remain unclear. Here we used the Drosophila steroidogenic organ, called the prothoracic gland (PG), to study regulatory mechanisms of MES, which is critical for the PG to upregulate biosynthesis of the steroid hormone ecdysone. We demonstrate that PG cells undergo MES through downregulation of mitotic cyclins, which is mediated by Fizzy-related (Fzr). Moreover, we performed a RNAi screen to further elucidate the regulatory mechanisms of MES, and identified the evolutionarily conserved chaperonin TCP-1 ring complex (TRiC) as a novel regulator of MES. Knockdown of TRiC subunits in the PG caused a prolonged mitotic period, probably due to impaired nuclear translocation of Fzr, which also caused loss of ecdysteroidogenic activity. These results indicate that TRiC supports proper MES and endocycle progression by regulating Fzr folding. We propose that TRiC-mediated protein quality control is a conserved mechanism supporting MES and endocycling, as well as subsequent terminal differentiation.
巻・号 15(4)
ページ e1008121
公開日 2019-4-1
DOI 10.1371/journal.pgen.1008121
PII PGENETICS-D-18-01663
PMID 31034473
PMC PMC6508744
MeSH Animals Cell Cycle* / genetics Chaperonins / metabolism* Drosophila / physiology* Drosophila Proteins / genetics Drosophila Proteins / metabolism Ecdysone / biosynthesis Larva Mitosis* / genetics Models, Biological Protein Transport RNA Interference
IF 5.224
引用数 1
リソース情報
ショウジョウバエ 10440R-3 HMS00174  HMS00451  HMJ21628  HMC03366  10664R-3 10689R-2 10691R-1 10712R-2 HMS00150  10840R-3 HMJ22671  HMJ23946  1088R-1 10903R-1 10908R-1 HMJ23868  10122R-1 HMS03370  10939R-3 10174R-4 10955R-3 10198R-1 10978R-3 11015R-3 10225R-4 11030R-3 10267R-2 11084R-2 10279R-1 1109R-1 10315R-1 HMS00850  HMS00175  11094R-1 HMJ21204  11132R-1 10435R-3 11180R-1 1044R-3 11188R-1 11207R-1 HMJ22921  11246R-3 11274R-1 11295R-4 11306R-1 11314R-1 11325R-1 1133R-7 11417R-3 11466R-4 1147R-1 HMJ23415  11555R-2 3058R-2 3071R-2 HMJ22254  31044R-1 HMS00493  31220R-2 HMJ21754  4155R-1 31313R-9 3139R-2 HMJ22430  31716R-1 11606R-2 3173R-2 1163R-2 31748R-1 3180R-3 HMJ30148  11859R-3 HMJ23393  31917R-2 11888R-1 HMJ23923  11901R-2 HMJ23850  HMJ21537  11943R-2 HMJ21554  11979R-1 3208R-1 11981R-3 32104R-1 HMS00097  11003R-3 32137R-2 11990R-2 HMS00445  HMS00122  32211R-7 12007R-2 8037R-1 3228R-1 12015R-3 3234R-2 12019R-1 HMJ23354  12082R-2 3246R-1 12085R-2 HMJ21831  12101R-3 2551R-2 3284R-3 12127R-1 17043R-4 HMS00763  3298R-1 HMS02703  6926R-2 HMC02909  3229R-1 HMJ21600  HMJ22343  3329R-1 2905R-7 HMJ23962  HMJ30077  12202R-2 HMJ30320  15388R-4 12209R-1 15509R-2 HMS00843  HMJ21896  HMS00665  3412R-1 HMJ22056  12325R-3 1884R-1 1234R-1 15610R-3 HMS00661  HMJ23317  3539R-3 12390R-2 HMJ23203  12749R-1 3582R-2 12752R-2 HMS00589  3644R-1 12759R-4 3704R-3 12785R-1 HMS00587  12864R-2 HMJ24042  12975R-2 3780R-1 3806R-3 HMS00206  3817R-1 13097R-3 HMS00837  HMJ23768  3821R-1 1324R-1 3847R-3 3874R-1 HMJ23572  3906R-1 HMJ24062  HMS00338  HMJ21045  4003R-1 4057R-2 4071R-1 4097R-2 4103R-3 4152R-1 13333R-2 4157R-2 13391R-4 4166R-1 HMS00879  4202R-3 4214R-1 13419R-3 31348R-1 HMJ23635  HMJ23223  13625R-1 HMC03255  HMJ02071  4258R-2 HMS00075  13626R-2 HMS00310  13692R-3 13746R-2 13758R-3 13773R-1 HMJ21397  14021R-3 HMS02389  14026R-3 4316R-1 HMJ22630  HMJ22351  16922R-2 14181R-3 4380R-1 14210R-1 1903R-2 4457R-2 14230R-1 4491R-1 HMS00196  1630R-2 14424R-3 4510R-2 4539R-1 14543R-7 HMC03176  14637R-2 4561R-1 14804R-4 4579R-3 4602R-2 HMJ22843  4634R-2 HMS00245  4659R-1 4673R-2 HMS01184  14805R-1 4738R-3 14992R-1 4817R-2 4825R-1 15019R-1 4843R-2 15064R-2 4849R-3 15067R-4 4854R-2 4875R-4 HMJ21309  4881R-1 1519R-2 4901R-3 15287R-1 4975R-1 5018R-2 HMJ30070  HMS02623  15481R-4 5064R-3 1554R-1 5079R-2 1560R-1 HMS01542  5123R-2 HMS00145  5179R-2 15811R-4 5277R-1 1603R-1 5289R-3 HMJ21050  1666R-2 5366R-3 HMC03308  5374R-2 16885R-7 5378R-1 5380R-3 16916R-4 HMJ22719  HMS00157  5432R-4 1703R-1 5444-1R-4 5484R-1 HMS00285  5519R-1 5524R-2 5525R-3 5553R-1 5596R-2 17083R-2 HMJ21931  17166R-2 HMJ30041  5649R-3 HMJ22988  HMJ21495  5706R-3 1746R-3 HMS00326  HMJ23796  5758R-2 17524R-2 5786R-4 17603R-2 5800R-3 5824R-1 17611R-1 5825R-3 17646R-2 5838R-4 HMS00721  17652R-3 5861R-2 31990R-2 HMS00994  HMJ21192  5939R-2 5970R-2 17932R-1 HMS00525  HMJ22113  5999R-1 HMS00631  HMJ21401  6095R-1 18130R-1 6121R-1 18174R-3 6141R-1 6155R-3 1828R-2 6223R-2 18292R-2 HMJ23052  1388R-1 6292R-3 6294R-1 18495R-3 6322R-1 6335R-2 6349R-3 HMS01541  6413R-2 6501R-3 6546R-1 6711R-2 18497R-1 HMJ22412  6738R-1 HMS00074  6752R-1 1873R-4 6756R-1 HMC03313  6764R-2 6778R-1 18870R-2 1965R-2 HMJ23056  2023R-1 6842R-3 HMJ21460  6856R-1 2054R-1 6876R-1 HMJ21823  HMJ22947  6937R-3 HMC02888  HMJ24037  2095R-1 6987R-2 7006R-2 2098R-2 7033R-1 2100R-1 7034R-1 2173R-2 7035R-3 2252R-2 7108-1R-1 7146R-2 2260R-1 HMJ30275  2263R-1 7241R-2 7275R-4 2522R-3 HMS00118  2637R-1 2685R-7 7338R-1 7339R-1 2691R-2 HMJ22403  7380R-2 7391R-2 7398R-1 7420R-1 HMC03144  7427R-2 7467R-3 2711R-3 7487R-3 7516R-1 2807R-3 7584R-4 2843R-1 HMS00155  HMJ23844  7654R-1 7697R-1 2875R-3 7725R-1 HMS00166  7728R-2 2970R-1 7757R-3 7776R-2 HMS00067  HMJ30215  3029R-2 7843R-1 HMJ23047  7845R-2 7885R-3 3033R-3 7927R-1 7957R-3 7962R-1 7989R-1 7993R-4 HMJ22816  8025R-2 8048R-2 8064R-1 8068R-1 8112R-3 8145R-1 8174R-3 8190R-2 8241R-4 8258R-2 HMS00917  8309R-1 HMJ23427  8370R-2 HMS00139  8395R-3 8426R-3 HMS00361  8431R-1 8432R-3 8439R-1 8454R-1 8545R-1 8580R-2 8605R-2 8610R-1 HMJ21015  HMC02981  HMS00561  8728R-2 HMS00274  8759R-1 8801R-3 8844R-2 HMJ21481  8977R-1 HMC03194  9107R-2 9131R-1 HMS00159  HMS00634  9246R-1 9273R-1 9281R-2 HMS00128  9298Rb-3 9305R-1 9327R-1 9378R-1 9468R-1 9498R-1 9512R-2 HMJ23473  9591R-2 9630R-2 9633R-1 HMS00134  9676R-3 9680R-2 9696R-1 9710R-1 9735R-6 9750R-1 9768R-2 HMS00940  HMS00645  9799R-3 9834R-3 9853R-2 HMS00252  HMJ23419  HMJ02126  9972R-1 9984R-1 HMJ23059  9999R-1 5940R-1 3510R-1 HMS00639 HMS01190 8351R-3 DGRC#111943 DGRC#111690