RRC ID 55611
Author Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.
Title Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.
Journal PLoS Genet
Abstract Endocycle is a commonly observed cell cycle variant through which cells undergo repeated rounds of genome DNA replication without mitosis. Endocycling cells arise from mitotic cells through a switch of the cell cycle mode, called the mitotic-to-endocycle switch (MES), to initiate cell growth and terminal differentiation. However, the underlying regulatory mechanisms of MES remain unclear. Here we used the Drosophila steroidogenic organ, called the prothoracic gland (PG), to study regulatory mechanisms of MES, which is critical for the PG to upregulate biosynthesis of the steroid hormone ecdysone. We demonstrate that PG cells undergo MES through downregulation of mitotic cyclins, which is mediated by Fizzy-related (Fzr). Moreover, we performed a RNAi screen to further elucidate the regulatory mechanisms of MES, and identified the evolutionarily conserved chaperonin TCP-1 ring complex (TRiC) as a novel regulator of MES. Knockdown of TRiC subunits in the PG caused a prolonged mitotic period, probably due to impaired nuclear translocation of Fzr, which also caused loss of ecdysteroidogenic activity. These results indicate that TRiC supports proper MES and endocycle progression by regulating Fzr folding. We propose that TRiC-mediated protein quality control is a conserved mechanism supporting MES and endocycling, as well as subsequent terminal differentiation.
Volume 15(4)
Pages e1008121
Published 2019-4-1
DOI 10.1371/journal.pgen.1008121
PII PGENETICS-D-18-01663
PMID 31034473
PMC PMC6508744
MeSH Animals Cell Cycle* / genetics Chaperonins / metabolism* Drosophila / physiology* Drosophila Proteins / genetics Drosophila Proteins / metabolism Ecdysone / biosynthesis Larva Mitosis* / genetics Models, Biological Protein Transport RNA Interference
IF 5.224
Times Cited 1
Resource
Drosophila 10122R-1 10174R-4 10198R-1 10225R-4 10267R-2 10279R-1 10315R-1 HMS00175  HMJ21204  10435R-3 1044R-3 10440R-3 HMS00174  HMS00451  HMJ21628  HMC03366  10664R-3 10689R-2 10691R-1 10712R-2 HMS00150  10840R-3 HMJ22671  HMJ23946  1088R-1 10903R-1 10908R-1 HMJ23868  HMS03370  10939R-3 10955R-3 10978R-3 11015R-3 11030R-3 11084R-2 1109R-1 HMS00850  11094R-1 11132R-1 11180R-1 11188R-1 11207R-1 HMJ22921  11246R-3 11274R-1 11295R-4 11306R-1 11314R-1 11325R-1 1133R-7 11417R-3 11466R-4 1147R-1 11555R-2 HMJ22430  11606R-2 1163R-2 HMJ30148  11859R-3 11888R-1 11901R-2 11943R-2 11979R-1 11981R-3 HMS00097  11990R-2 HMS00122  12007R-2 12015R-3 12019R-1 12082R-2 12085R-2 12101R-3 12127R-1 HMS00763  HMJ30077  12202R-2 12209R-1 HMS00843  HMS00665  12325R-3 1234R-1 HMJ23317  12390R-2 12749R-1 12752R-2 12759R-4 12785R-1 12864R-2 12975R-2 HMS00206  13097R-3 HMJ23768  1324R-1 HMJ23572  HMJ24062  13333R-2 13391R-4 HMS00879  13419R-3 HMJ23635  13625R-1 13626R-2 13692R-3 13746R-2 13758R-3 13773R-1 HMJ21397  14021R-3 14026R-3 HMJ22351  14181R-3 14210R-1 14230R-1 HMS00196  14424R-3 14543R-7 14637R-2 14804R-4 14805R-1 14992R-1 15019R-1 15064R-2 15067R-4 HMJ21309  1519R-2 15287R-1 HMJ30070  15481R-4 1554R-1 1560R-1 HMS00145  15811R-4 1603R-1 1666R-2 HMC03308  16885R-7 16916R-4 HMS00157  1703R-1 17083R-2 HMJ21931  17166R-2 HMJ30041  HMJ22988  1746R-3 HMJ23796  17524R-2 17603R-2 17611R-1 17646R-2 17652R-3 31990R-2 HMJ21192  17932R-1 HMJ22113  HMS00631  18130R-1 18174R-3 1828R-2 18292R-2 1388R-1 18495R-3 18497R-1 HMS00074  1873R-4 HMC03313  18870R-2 1965R-2 2023R-1 HMJ21460  2054R-1 HMJ22947  HMC02888  2095R-1 2098R-2 2100R-1 2173R-2 2252R-2 2260R-1 2263R-1 2522R-3 2637R-1 2685R-7 2691R-2 2711R-3 2807R-3 2843R-1 HMJ23844  2875R-3 HMS00166  2970R-1 HMS00067  3029R-2 HMJ23047  3033R-3 HMJ23415  3058R-2 3071R-2 HMJ22254  31044R-1 HMS00493  31220R-2 HMJ21754  4155R-1 31313R-9 3139R-2 31716R-1 3173R-2 31748R-1 3180R-3 HMJ23393  31917R-2 HMJ23923  HMJ23850  HMJ21537  HMJ21554  3208R-1 32104R-1 11003R-3 32137R-2 HMS00445  32211R-7 8037R-1 3228R-1 3234R-2 HMJ23354  3246R-1 HMJ21831  2551R-2 3284R-3 17043R-4 3298R-1 HMS02703  6926R-2 HMC02909  3229R-1 HMJ21600  HMJ22343  3329R-1 2905R-7 HMJ23962  HMJ30320  15388R-4 15509R-2 HMJ21896  3412R-1 HMJ22056  1884R-1 15610R-3 HMS00661  3539R-3 HMJ23203  3582R-2 HMS00589  3644R-1 3704R-3 HMS00587  HMJ24042  3780R-1 3806R-3 3817R-1 HMS00837  3821R-1 3847R-3 3874R-1 3906R-1 HMS00338  HMJ21045  4003R-1 4057R-2 4071R-1 4097R-2 4103R-3 4152R-1 4157R-2 4166R-1 4202R-3 4214R-1 31348R-1 HMJ23223  HMC03255  HMJ02071  4258R-2 HMS00075  HMS00310  HMS02389  4316R-1 HMJ22630  16922R-2 4380R-1 1903R-2 4457R-2 4491R-1 1630R-2 4510R-2 4539R-1 HMC03176  4561R-1 4579R-3 4602R-2 HMJ22843  4634R-2 HMS00245  4659R-1 4673R-2 HMS01184  4738R-3 4817R-2 4825R-1 4843R-2 4849R-3 4854R-2 4875R-4 4881R-1 4901R-3 4975R-1 5018R-2 HMS02623  5064R-3 5079R-2 HMS01542  5123R-2 5179R-2 5277R-1 5289R-3 HMJ21050  5366R-3 5374R-2 5378R-1 5380R-3 HMJ22719  5432R-4 5444-1R-4 5484R-1 HMS00285  5519R-1 5524R-2 5525R-3 5553R-1 5596R-2 5649R-3 HMJ21495  5706R-3 HMS00326  5758R-2 5786R-4 5800R-3 5824R-1 5825R-3 5838R-4 HMS00721  5861R-2 HMS00994  5939R-2 5970R-2 HMS00525  5999R-1 HMJ21401  6095R-1 6121R-1 6141R-1 6155R-3 6223R-2 HMJ23052  6292R-3 6294R-1 6322R-1 6335R-2 6349R-3 HMS01541  6413R-2 6501R-3 6546R-1 6711R-2 HMJ22412  6738R-1 6752R-1 6756R-1 6764R-2 6778R-1 HMJ23056  6842R-3 6856R-1 6876R-1 HMJ21823  6937R-3 HMJ24037  6987R-2 7006R-2 7033R-1 7034R-1 7035R-3 7108-1R-1 7146R-2 HMJ30275  7241R-2 7275R-4 HMS00118  7338R-1 7339R-1 HMJ22403  7380R-2 7391R-2 7398R-1 7420R-1 HMC03144  7427R-2 7467R-3 7487R-3 7516R-1 7584R-4 HMS00155  7654R-1 7697R-1 7725R-1 7728R-2 7757R-3 7776R-2 HMJ30215  7843R-1 7845R-2 7885R-3 7927R-1 7957R-3 7962R-1 7989R-1 7993R-4 HMJ22816  8025R-2 8048R-2 8064R-1 8068R-1 8112R-3 8145R-1 8174R-3 8190R-2 8241R-4 8258R-2 HMS00917  8309R-1 HMJ23427  8370R-2 HMS00139  8395R-3 8426R-3 HMS00361  8431R-1 8432R-3 8439R-1 8454R-1 8545R-1 8580R-2 8605R-2 8610R-1 HMJ21015  HMC02981  HMS00561  8728R-2 HMS00274  8759R-1 8801R-3 8844R-2 HMJ21481  8977R-1 HMC03194  9107R-2 9131R-1 HMS00159  HMS00634  9246R-1 9273R-1 9281R-2 HMS00128  9298Rb-3 9305R-1 9327R-1 9378R-1 9468R-1 9498R-1 9512R-2 HMJ23473  9591R-2 9630R-2 9633R-1 HMS00134  9676R-3 9680R-2 9696R-1 9710R-1 9735R-6 9750R-1 9768R-2 HMS00940  HMS00645  9799R-3 9834R-3 9853R-2 HMS00252  HMJ23419  HMJ02126  9972R-1 9984R-1 HMJ23059  9999R-1 5940R-1 3510R-1 HMS00639 HMS01190 8351R-3 DGRC#111943 DGRC#111690