RRC ID 55704
著者 Tonoyama Y, Tsukada M, Imai Y, Sanada M, Aota S, Oka G, Sugiura S, Hori N, Kawachi H, Shimizu Y, Shimizu N.
タイトル Establishment of a quantitative in vivo method for estimating adipose tissue volumes and the effects of dietary soy sauce oil on adipogenesis in medaka, Oryzias latipes.
ジャーナル PLoS One
Abstract Adipose tissue, which is conserved in higher eukaryotes, plays central roles in controlling the body's energy balance, including excess energy storage and energy expenditure during starvation. In adipogenesis, intranuclear receptor, peroxisome proliferator-activated receptor gamma (PPARγ) is a key molecule, and PPARγ agonists can promote adipogenesis. Many studies on the in vitro screening of PPARγ agonists with compounds derived from various materials have been reported; however, in vivo assays for quick examination of these feeding effects have not been established. In this study, we developed a technique using a lipophilic fluorescent reagent, Nile red to quantitatively estimate the adipose tissue volumes by using Japanese rice fish, medaka (Oryzias latipes) and studied effects of dietary soy sauce oil (SSO), which is a discarded by-product from Japanese traditional food and is known to have PPARγ-agonistic activity, on adipogenesis. We found that SSO feeding increased the adipose tissue volumes, and the expression levels of adipogenesis-related genes increased in these medaka larvae. These results suggest that SSO feeding increases the adipose tissue volumes through adipogenesis promotion by PPARγ-agonistic activity in medaka, and medaka is a powerful model for studying adipogenesis. Furthermore, our study also demonstrates the availability of SSO as a dietary additive for farmed fish.
巻・号 13(10)
ページ e0205888
公開日 2018-10-18
DOI 10.1371/journal.pone.0205888
PII PONE-D-18-01781
PMID 30335858
PMC PMC6193695
MeSH Activin Receptors, Type I / genetics Activin Receptors, Type I / metabolism Adipogenesis / drug effects* Adipogenesis / genetics Adiponectin / genetics Adiponectin / metabolism Adipose Tissue / drug effects* Adipose Tissue / metabolism Animal Feed / analysis* Animals Animals, Genetically Modified Aquaculture Diet / methods Dietary Fats, Unsaturated / administration & dosage* Energy Metabolism / drug effects Energy Metabolism / genetics Fatty Acid-Binding Proteins / genetics Fatty Acid-Binding Proteins / metabolism Fish Proteins / genetics Fish Proteins / metabolism Fluorescent Dyes / administration & dosage Fluorescent Dyes / chemistry Gene Expression Regulation Genes, Reporter Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Larva / drug effects* Larva / genetics Larva / growth & development Larva / metabolism Lipid Metabolism / drug effects Lipid Metabolism / genetics Oryzias / genetics* Oryzias / growth & development Oryzias / metabolism Oxazines / administration & dosage Oxazines / chemistry PPAR gamma / agonists PPAR gamma / genetics* PPAR gamma / metabolism Soy Foods / analysis
IF 2.776
引用数 0
リソース情報
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