論文 - 詳細
| RRC ID | 55717 |
|---|---|
| 著者 | Shiota Y, Sakurai T, Daimon T, Mitsuno H, Fujii T, Matsuyama S, Sezutsu H, Ishikawa Y, Kanzaki R. |
| タイトル | In vivo functional characterisation of pheromone binding protein-1 in the silkmoth, Bombyx mori. |
| ジャーナル | Sci Rep |
| Abstract |
Male moths detect sex pheromones emitted by conspecific females with high sensitivity and specificity by the olfactory sensilla on their antennae. Pheromone binding proteins (PBPs) are highly enriched in the sensillum lymph of pheromone sensitive olfactory sensilla and are supposed to contribute to the sensitivity and selectivity of pheromone detection in moths. However, the functional role of PBPs in moth sex pheromone detection in vivo remains obscure. In the silkmoth, Bombyx mori, female moths emit bombykol as a single attractive sex pheromone component along with a small amount of bombykal that negatively modulates the behavioural responses to bombykol. A pair of olfactory receptor neurons, specifically tuned to bombykol or bombykal, co-localise in the trichodeum sensilla, the sensillum lymph of which contains a single PBP, namely, BmPBP1. We analysed the roles of BmPBP1 using BmPBP1-knockout silkmoth lines generated by transcription activator-like effector nuclease-mediated gene targeting. Electroantennogram analysis revealed that the peak response amplitudes of BmPBP1-knockout male antennae to bombykol and bombykal were significantly reduced by a similar percentage when compared with those of the wild-type males. Our results indicate that BmPBP1 plays a crucial role in enhancing the sensitivity, but not the selectivity, of sex pheromone detection in silkmoths. |
| 巻・号 | 8(1) |
| ページ | 13529 |
| 公開日 | 2018-9-10 |
| DOI | 10.1038/s41598-018-31978-2 |
| PII | 10.1038/s41598-018-31978-2 |
| PMID | 30202026 |
| PMC | PMC6131395 |
| MeSH | Alkadienes / metabolism Animals Animals, Genetically Modified Bombyx / physiology* Carrier Proteins / genetics Carrier Proteins / metabolism* Fatty Alcohols / metabolism Female Gene Knockdown Techniques Insect Proteins / genetics Insect Proteins / metabolism* Male Olfactory Receptor Neurons / metabolism Sensilla / cytology Sensilla / metabolism Sex Attractants / metabolism* Sexual Behavior, Animal / physiology* |
| IF | 4.011 |
| 引用数 | 10 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
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