RRC ID 55741
著者 Sugiyama Y, Oishi T, Yamashita A, Murata Y, Yamamoto T, Takashima I, Isa T, Higo N.
タイトル Neuronal and microglial localization of secreted phosphoprotein 1 (osteopontin) in intact and damaged motor cortex of macaques.
ジャーナル Brain Res
Abstract We previously reported that mRNA encoding secreted phosphoprotein 1 (SPP1), also known as osteopontin, is preferentially expressed in large neurons in layer V of the macaque motor cortex, most of which are presumed to be corticospinal tract neurons. As a first step to elucidating the cellular function of SPP1 in macaque neurons, we examined the localization of SPP1 in the primary motor cortex (M1) of the macaque by using immunohistochemistry. SPP1 immunoreactivity was found to be localized in the cell bodies of neurons, but not outside the cells, indicating that SPP1 was not secreted from these neurons. The results of electron microscope analysis and double-labeling analysis with marker proteins suggested that SPP1 was localized in the mitochondria of neurons. The distributions of SPP1 in the neurons corresponded to those of integrin αV, a putative receptor for SPP1. The distribution of SPP1 was also investigated in macaques whose M1 had been lesioned. We found that SPP1 was secreted by proliferated microglia in the lesioned area. Double-labeling analysis indicated that SPP1 immunoreactivity in the microglia was colocalized with CD44, another putative receptor for SPP1. Success rates in the small-object-retrieval task were positively correlated with SPP1 immunoreactivity in the neurons in the perilesional area. SPP1 has multiple roles in the macaque motor cortex, and it may be a key protein during recovery of hand movement after brain damage.
巻・号 1714
ページ 52-64
公開日 2019-7-1
DOI 10.1016/j.brainres.2019.02.021
PII S0006-8993(19)30105-2
PMID 30790559
MeSH Animals Female Hyaluronan Receptors / immunology In Situ Hybridization / methods Macaca mulatta Male Microglia / metabolism Motor Cortex / metabolism* Motor Cortex / pathology Neurons / metabolism* Osteopontin / genetics Osteopontin / metabolism* Osteopontin / physiology Pyramidal Tracts / metabolism RNA, Messenger / metabolism
IF 2.929
引用数 0
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