Reference - Detail
| RRC ID | 55794 |
|---|---|
| Author | Daspute AA, Yunxuan X, Gu M, Kobayashi Y, Wagh S, Panche A, Koyama H. |
| Title | Agrobacterium rhizogenes-mediated hairy roots transformation as a tool for exploring aluminum-responsive genes function. |
| Journal | Future Sci OA |
| Abstract |
Aim:To develop a useful alternative approach to evaluate the gene function in hairy roots. Methods:Arabidopsis and tobacco (wild-type or mutant) were a host for Agrobacterium rhizogenes transformation. Results:The hairy roots formation efficiency ranged from 53 to 98% in tobacco and 53 to 66% in Arabidopsis. Hairy and intact roots showed similar gene expression pattern in response to salt and aluminum stress. Genomic polymerase chain reaction and fluorescent images showed high rate (>80%) of co-integration of T-DNAs and uniform cell transformation without use of any antibiotic selection. Whole processes of hairy roots were completed within 1 month after the infection of Agrobacterium. Conclusion:Aluminum-responsive orthologous gene function could be evaluated by NtSTOP1-KD and Atstop1 as a host for hairy roots transformation. |
| Volume | 5(3) |
| Pages | FSO364 |
| Published | 2019-2-8 |
| DOI | 10.4155/fsoa-2018-0065 |
| PMID | 30906565 |
| PMC | PMC6426172 |
| Times Cited | 1 |
| Resource | |
| Arabidopsis / Cultured plant cells, genes | psi00011 |