Reference - Detail
| RRC ID | 55814 |
|---|---|
| Author | Yura T, Miyazaki R, Fujiwara K, Ito K, Chiba S, Mori H, Akiyama Y. |
| Title | Heat shock transcription factor σ32 defective in membrane transport can be suppressed by transposon insertion into genes encoding a restriction enzyme subunit or a putative autotransporter in Escherichia coli. |
| Journal | Genes Genet Syst |
| Abstract |
Heat shock transcription factor σ32 of Escherichia coli plays a major role in protein homeostasis and requires membrane localization for regulation. We here report that a strongly deregulated I54N-σ32 mutant defective in association with the membrane can be phenotypically suppressed by Tn5 insertion into the mcrC or ydbA2 gene, encoding a restriction enzyme subunit or part of a putative autotransporter, respectively. The suppression is specific for mutant I54N-σ32 and reduces its activity but not its abundance or stability. Moreover, the deregulated phenotype of I54N-σ32 is effectively suppressed by a plasmid carrying the same mcrC::Tn5 mutation. In contrast, deletion of the mcrC or ydbA2 gene hardly affects I54N-σ32 activity. These results, taken together, suggest that the truncated form of McrC (and presumably also of YdbA2) protein produced by the Tn5 insertion interacts specifically with I54N-σ32 to reduce its activity without substantially affecting its amount or stability. |
| Volume | 93(6) |
| Pages | 229-235 |
| Published | 2019-1-19 |
| DOI | 10.1266/ggs.18-00040 |
| PMID | 30531155 |
| MeSH | DNA Restriction Enzymes / genetics DNA Restriction Enzymes / metabolism* DNA Transposable Elements Escherichia coli / genetics Escherichia coli / metabolism Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Heat Shock Transcription Factors / genetics* Heat Shock Transcription Factors / metabolism Recombination, Genetic Suppression, Genetic* |
| IF | 0.859 |
| Times Cited | 1 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes E. coli | ∆mcrC, ∆ydbA2 |