| RRC ID |
55814
|
| Author |
Yura T, Miyazaki R, Fujiwara K, Ito K, Chiba S, Mori H, Akiyama Y.
|
| Title |
Heat shock transcription factor σ32 defective in membrane transport can be suppressed by transposon insertion into genes encoding a restriction enzyme subunit or a putative autotransporter in Escherichia coli.
|
| Journal |
Genes Genet Syst
|
| Abstract |
Heat shock transcription factor σ32 of Escherichia coli plays a major role in protein homeostasis and requires membrane localization for regulation. We here report that a strongly deregulated I54N-σ32 mutant defective in association with the membrane can be phenotypically suppressed by Tn5 insertion into the mcrC or ydbA2 gene, encoding a restriction enzyme subunit or part of a putative autotransporter, respectively. The suppression is specific for mutant I54N-σ32 and reduces its activity but not its abundance or stability. Moreover, the deregulated phenotype of I54N-σ32 is effectively suppressed by a plasmid carrying the same mcrC::Tn5 mutation. In contrast, deletion of the mcrC or ydbA2 gene hardly affects I54N-σ32 activity. These results, taken together, suggest that the truncated form of McrC (and presumably also of YdbA2) protein produced by the Tn5 insertion interacts specifically with I54N-σ32 to reduce its activity without substantially affecting its amount or stability.
|
| Volume |
93(6)
|
| Pages |
229-235
|
| Published |
2019-1-19
|
| DOI |
10.1266/ggs.18-00040
|
| PMID |
30531155
|
| MeSH |
DNA Restriction Enzymes / genetics
DNA Restriction Enzymes / metabolism*
DNA Transposable Elements
Escherichia coli / genetics
Escherichia coli / metabolism
Escherichia coli Proteins / genetics
Escherichia coli Proteins / metabolism*
Heat Shock Transcription Factors / genetics*
Heat Shock Transcription Factors / metabolism
Recombination, Genetic
Suppression, Genetic*
|
| IF |
0.859
|
| Times Cited |
1
|
| Resource |
| Prokaryotes E. coli |
∆mcrC, ∆ydbA2 |
