| RRC ID |
55821
|
| 著者 |
Arita-Morioka KI, Yamanaka K, Mizunoe Y, Tanaka Y, Ogura T, Sugimoto S.
|
| タイトル |
Inhibitory effects of Myricetin derivatives on curli-dependent biofilm formation in Escherichia coli.
|
| ジャーナル |
Sci Rep
|
| Abstract |
Biofilms are well-organised communities of microbes embedded in a self-produced extracellular matrix (e.g., curli amyloid fibers) and are associated with chronic infections. Therefore, development of anti-biofilm drugs is important to combat with these infections. Previously, we found that flavonol Myricetin inhibits curli-dependent biofilm formation by Escherichia coli (IC50 = 46.2 μM). In this study, we tested activities of seven Myricetin-derivatives to inhibit biofilm formation by E. coli K-12 in liquid culture. Among them, only Epigallocatechin gallate (EGCG), a major catechin in green tea, inhibited biofilm formation of K-12 (IC50 = 5.9 μM) more efficiently than Myricetin. Transmission electron microscopy and immunoblotting analyses demonstrated that EGCG prevented curli production by suppressing the expression of curli-related proteins. Quantitative RT-PCR analysis revealed that the transcripts of csgA, csgB, and csgD were significantly reduced in the presence of EGCG. Interestingly, the cellular level of RpoS, a stationary-phase specific alternative sigma factor, was reduced in the presence of EGCG, whereas the rpoS transcript was not affected. Antibiotic-chase experiments and genetic analyses revealed that EGCG accelerated RpoS degradation by ATP-dependent protease ClpXP in combination with its adaptor RssB. Collectively, these results provide significant insights into the development of drugs to treat chronic biofilm-associated infections.
|
| 巻・号 |
8(1)
|
| ページ |
8452
|
| 公開日 |
2018-5-31
|
| DOI |
10.1038/s41598-018-26748-z
|
| PII |
10.1038/s41598-018-26748-z
|
| PMID |
29855532
|
| PMC |
PMC5981455
|
| MeSH |
Bacterial Proteins / genetics
Bacterial Proteins / metabolism*
Biofilms / drug effects*
Biofilms / growth & development
Catechin / analogs & derivatives
Catechin / pharmacology
Escherichia coli / physiology*
Flavonoids / chemistry
Flavonoids / pharmacology*
Gene Expression Regulation, Bacterial / drug effects
Sigma Factor / genetics
Sigma Factor / metabolism
|
| IF |
4.011
|
| 引用数 |
8
|
| リソース情報 |
| 原核生物(大腸菌) |
BW25113, ΔcsgA, ΔcsgB, ΔcsgD, ΔcsgG, ΔrpoS, ΔclpA, ΔclpX, ΔclpP, Δlon, ΔhslU, ΔhslV, ΔrssB, and ΔiraP |
