Reference - Detail
|Author||Liu L, Liu Y, Zhang G, Ge Y, Fan X, Lin F, Wang J, Zheng H, Xie X, Zeng X, Chen PR.|
|Title||Genetically Encoded Chemical Decaging in Living Bacteria.|
We report the genetically encoded chemical decaging strategy for protein activation in living bacterial cells. In contrast to the metabolically labile photocaging groups inside Escherichia coli, our chemical decaging strategy that relies on the inverse electron-demand Diels-Alder (iDA) reaction is compatible with the intracellular environment of bacteria, which can be a general tool for gain-of-function study of a given protein in prokaryotic systems. By applying this strategy for in situ activation of the indole-producing enzyme TnaA, we built an orthogonal and chemically inducible indole production pathway inside E. coli cells, which revealed the role of indole in bacterial antibiotic tolerance.
|MeSH||Cyclooctanes Enzyme Activation / radiation effects Escherichia coli Proteins / chemistry* Escherichia coli Proteins / genetics Escherichia coli Proteins / radiation effects Green Fluorescent Proteins / genetics Indoles / metabolism Mutagenesis, Site-Directed Nitrobenzenes Photochemistry Tryptophanase / chemistry* Tryptophanase / genetics Tryptophanase / radiation effects Ultraviolet Rays|
|Prokaryotes E. coli||BW25113|