論文 - 詳細
| RRC ID | 55848 |
|---|---|
| 著者 | Fontaine BM, Martin KS, Garcia-Rodriguez JM, Jung C, Briggs L, Southwell JE, Jia X, Weinert EE. |
| タイトル | RNase I regulates Escherichia coli 2',3'-cyclic nucleotide monophosphate levels and biofilm formation. |
| ジャーナル | Biochem J |
| Abstract |
Regulation of nucleotide and nucleoside concentrations is critical for faithful DNA replication, transcription, and translation in all organisms, and has been linked to bacterial biofilm formation. Unusual 2',3'-cyclic nucleotide monophosphates (2',3'-cNMPs) recently were quantified in mammalian systems, and previous reports have linked these nucleotides to cellular stress and damage in eukaryotes, suggesting an intriguing connection with nucleotide/nucleoside pools and/or cyclic nucleotide signaling. This work reports the first quantification of 2',3'-cNMPs in Escherichia coli and demonstrates that 2',3'-cNMP levels in E. coli are generated specifically from RNase I-catalyzed RNA degradation, presumably as part of a previously unidentified nucleotide salvage pathway. Furthermore, RNase I and 2',3'-cNMP levels are demonstrated to play an important role in controlling biofilm formation. This work identifies a physiological role for cytoplasmic RNase I and constitutes the first progress toward elucidating the biological functions of bacterial 2',3'-cNMPs. |
| 巻・号 | 475(8) |
| ページ | 1491-1506 |
| 公開日 | 2018-4-30 |
| DOI | 10.1042/BCJ20170906 |
| PII | BCJ20170906 |
| PMID | 29555843 |
| PMC | PMC6452634 |
| MeSH | Biofilms / growth & development* Escherichia coli / genetics Escherichia coli / growth & development Escherichia coli / metabolism* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Gene Expression Regulation, Bacterial Nucleotides, Cyclic / metabolism* RNA Stability RNA, Bacterial / genetics RNA, Bacterial / metabolism* RNA, Messenger / genetics RNA, Messenger / metabolism* Ribonuclease, Pancreatic / metabolism* Signal Transduction |
| IF | 4.331 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | BW25113 |