RRC ID 55898
Author Eto S, Goto M, Soga M, Kaneko Y, Uehara Y, Mizuta H, Era T.
Title Mesenchymal stem cells derived from human iPS cells via mesoderm and neuroepithelium have different features and therapeutic potentials.
Journal PLoS ONE
Abstract Mesenchymal stem cells (MSCs) isolated from adult human tissues are capable of proliferating in vitro and maintaining their multipotency, making them attractive cell sources for regenerative medicine. However, the availability and capability of self-renewal under current preparation regimes are limited. Induced pluripotent stem cells (iPSCs) now offer an alternative, similar cell source to MSCs. Herein, we established new methods for differentiating hiPSCs into MSCs via mesoderm-like and neuroepithelium-like cells. Both derived MSC populations exhibited self-renewal and multipotency, as well as therapeutic potential in mouse models of skin wounds, pressure ulcers, and osteoarthritis. Interestingly, the therapeutic effects differ between the two types of MSCs in the disease models, suggesting that the therapeutic effect depends on the cell origin. Our results provide valuable basic insights for the clinical application of such cells.
Volume 13(7)
Pages e0200790
Published 2018-7-25
DOI 10.1371/journal.pone.0200790
PII PONE-D-18-02372
PMID 30044827
PMC PMC6059447
MeSH Adipocytes / cytology Animals Cell Differentiation Disease Models, Animal Humans Induced Pluripotent Stem Cells / cytology* Male Mesenchymal Stem Cell Transplantation* Mesenchymal Stem Cells / cytology* Mesoderm / metabolism Mice Oligonucleotide Array Sequence Analysis Osteogenesis Regenerative Medicine / methods* Skin / metabolism
IF 2.766
Human and Animal Cells 201B7(HPS0063)