RRC ID 55928
Author Hirota Y, Nakagawa K, Isomoto K, Sakaki T, Kubodera N, Kamao M, Osakabe N, Suhara Y, Okano T.
Title Eldecalcitol is more effective in promoting osteogenesis than alfacalcidol in Cyp27b1-knockout mice.
Journal PLoS One
Abstract Calcium (Ca) absorption from the intestinal tract is promoted by active vitamin D (1α,25D3). Vitamin D not only promotes Ca homeostasis, but it also inhibits bone resorption and promotes osteogenesis, thus playing a role in the maintenance of normal bone metabolism. Because 1α,25D3 plays an important role in osteogenesis, vitamin D formulations, such as alfacalcidol (ALF) and eldecalcitol (ELD), are used for treating osteoporosis. While it is known that, in contrast to ALF, ELD is an active ligand that directly acts on bone, the reason for its superior osteogenesis effects is unknown. Cyp27b1-knockout mice (Cyp27b1-/-mice) are congenitally deficient in 1α,25D3 and exhibit marked hypocalcemia and high parathyroid hormone levels, resulting in osteodystrophy involving bone hypocalcification and growth plate cartilage hypertrophy. However, because the vitamin D receptor is expressed normally in Cyp27b1-/-mice, they respond normally to 1α,25D3. Accordingly, in Cyp27b1-/-mice, the pharmacological effects of exogenously administered active vitamin D derivatives can be analyzed without being affected by 1α,25D3. We used Cyp27b1-/-mice to characterize and clarify the superior osteogenic effects of ELD on the bone in comparison with ALF. The results indicated that compared to ALF, ELD strongly induces ECaC2, calbindin-D9k, and CYP24A1 in the duodenum, promoting Ca absorption and decreasing the plasma concentration of 1α,25D3, resulting in improved osteogenesis. Because bone morphological measurements demonstrated that ELD has stronger effects on bone calcification, trabecular formation, and cancellous bone density than ALF, ELD appears to be a more effective therapeutic agent for treating postmenopausal osteoporosis, in which cancellous bone density decreases markedly. By using Cyp27b1-/-mice, this study was the first to succeed in clarifying the osteogenic effect of ELD without any influence of endogenous 1α,25D3. Furthermore, ELD more strongly enhanced bone mineralization, trabecular proliferation, and cancellous bone density than did ALF. Thus, ELD is expected to show an effect on postmenopausal osteoporosis, in which cancellous bone mineral density decreases markedly. In the future, this study may enable the development of next-generation active vitamin D derivatives with higher affinity for bone than ELD.
Volume 13(10)
Pages e0199856
Published 2018-10-3
DOI 10.1371/journal.pone.0199856
PII PONE-D-18-17222
PMID 30281599
PMC PMC6169848
MeSH 25-Hydroxyvitamin D3 1-alpha-Hydroxylase / genetics* 25-Hydroxyvitamin D3 1-alpha-Hydroxylase / metabolism Animals Body Weight Calcium / metabolism Cell Line, Tumor Female Femur / diagnostic imaging Humans Hydroxycholecalciferols / pharmacology* Male Mice Mice, Knockout Osteogenesis / drug effects* Osteoporosis, Postmenopausal / drug therapy Receptors, Calcitriol Tibia / diagnostic imaging Transfection Vitamin D / analogs & derivatives* Vitamin D / metabolism Vitamin D / pharmacology
IF 2.776
Times Cited 1
Human and Animal Cells ATDC5(RCB0565)