論文 - 詳細
| RRC ID | 55971 |
|---|---|
| 著者 | Kurata A, Yamada M, Ohno SI, Inoue S, Hashimoto H, Fujita K, Takanashi M, Kuroda M. |
| タイトル | Expression level of microRNA-200c is associated with cell morphology in vitro and histological differentiation through regulation of ZEB1/2 and E-cadherin in gastric carcinoma. |
| ジャーナル | Oncol Rep |
| Abstract |
Scirrhous type gastric cancer is characterized by diffuse infiltration of poorly differentiated adenocarcinoma cells and poor prognosis. Although association of poorly differentiated histology with reduction in E-cadherin expression, as well as association of microRNA (miR)-200c with E-cadherin through regulation of ZEB1/2, has been reported, participation of miR-200c in gastric carcinogenesis is not fully understood. We used 6 cell lines originating from gastric cancers, and investigated levels of miR-200c along with its target mRNAs ZEB1/2 and E-cadherin by qRT-PCR. ZEB1 and E-cadherin protein expression was also assessed via western blotting. Furthermore, we investigated the expression levels of miR‑200c by in situ hybridization, along with the expression of ZEB1 and E-cadherin by immunohistochemistry, in 97 gastric adenocarcinoma tissues. Inverse correlation between miR‑200c and ZEB1 levels were obtained by qRT-PCR in cell lines (P<0.05). Cell lines with low miR-200c and high ZEB1 exhibited low E-cadherin expression in both qRT-PCR and western blotting, and exhibited spindle-shaped morphology, in contrast to round cell morphology in those cell lines with high miR-200c levels. Inverse correlations were also obtained between miR-200c and ZEB1 as well as between ZEB1 and E-cadherin levels in tissue samples (P<0.001). Cancer tissues with low miR-200c, high ZEB1, and low E-cadherin expression were associated with poorly differentiated histology, in contrast to tubular form in cancers with high miR-200c expression levels (P<0.001). Our data revealed that downregulation of miR-200c primarily regulated cell morphology by downregulation of E-cadherin through upregulation of ZEB1, leading to poorly differentiated histology in gastric cancer. |
| 巻・号 | 39(1) |
| ページ | 91-100 |
| 公開日 | 2018-1-1 |
| DOI | 10.3892/or.2017.6093 |
| PMID | 29138864 |
| PMC | PMC5783608 |
| MeSH | Adenocarcinoma, Scirrhous / genetics Adenocarcinoma, Scirrhous / metabolism Adenocarcinoma, Scirrhous / pathology* Adult Aged Aged, 80 and over Antigens, CD Cadherins / genetics Cadherins / metabolism* Cell Differentiation Cell Line, Tumor Epithelial-Mesenchymal Transition Female Gene Expression Regulation, Neoplastic Humans In Vitro Techniques Male MicroRNAs / genetics* Middle Aged Stomach Neoplasms / genetics Stomach Neoplasms / metabolism Stomach Neoplasms / pathology* Tumor Burden Zinc Finger E-box Binding Homeobox 2 / genetics Zinc Finger E-box Binding Homeobox 2 / metabolism* Zinc Finger E-box-Binding Homeobox 1 / genetics Zinc Finger E-box-Binding Homeobox 1 / metabolism* |
| IF | 3.041 |
| 引用数 | 7 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | H-111-TC(RCB1884) HGC-27(RCB0500) Kato III(RCB2088) MKN1(RCB1003) MKN45(RCB1001) NUGC-4(RCB1939) |