論文 - 詳細
| RRC ID | 55982 |
|---|---|
| 著者 | Razi MA, Wakabayashi R, Tahara Y, Goto M, Kamiya N. |
| タイトル | Genipin-stabilized caseinate-chitosan nanoparticles for enhanced stability and anti-cancer activity of curcumin. |
| ジャーナル | Colloids Surf B Biointerfaces |
| Abstract |
Nanoparticles formed by the assembly of protein and polysaccharides are of great interest for the delivery of hydrophobic molecules. Herein, the formation of genipin-crosslinked nanoparticles from caseinate (CS) and chitosan (CH) is reported for the delivery of curcumin, a polyphenolic compound from turmeric, to cells. Genipin-crosslinked CS-CH nanoparticles (G-CCNPs) having a diameter of ∼250 nm and a low polydispersity index showed excellent stability over a wide pH range, as indicated by dynamic light scattering and transmission electron microscopic measurements. Cellular uptake of curcumin loaded into G-CCNPs by HeLa cells was improved, as measured by confocal laser scanning microscopy (CLSM) and fluorescence-activated cell-sorting analysis. Cell proliferation assays indicated that G-CCNPs were nontoxic and that curcumin's anticancer activity in vitro was also improved by G-CCNPs. Stability of curcumin at neutral pH was enhanced by G-CCNPs. CLSM study revealed that G-CCNPs were poorly internalized by HeLa cells, possibly because of strong cell membrane interactions and a negative zeta potential. Overall, our results suggested that the enhanced curcumin cytotoxicity might be associated with the enhanced stability of curcumin by G-CCNPs and free curcumin released from G-CCNPs into the cell. These biocompatible NPs might be suitable carriers for enhancing curcumin's therapeutic potential. |
| 巻・号 | 164 |
| ページ | 308-315 |
| 公開日 | 2018-4-1 |
| DOI | 10.1016/j.colsurfb.2018.01.041 |
| PII | S0927-7765(18)30049-3 |
| PMID | 29413610 |
| MeSH | Antineoplastic Agents / pharmacology* Caseins / chemistry* Cell Death / drug effects Chitosan / chemistry* Curcumin / pharmacology* Drug Liberation Fluorescence HeLa Cells Humans Iridoids / chemistry* Nanoparticles / chemistry* Nanoparticles / ultrastructure Particle Size |
| IF | 3.973 |
| 引用数 | 12 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa |