論文 - 詳細
| RRC ID | 55 |
|---|---|
| 著者 | Nakatsukasa E, Kashiwazaki N, Takizawa A, Shino M, Kitada K, Serikawa T, Hakamata Y, Kobayashi E, Takahashi R, Ueda M, Nakashima T, Nakagata N. |
| タイトル | Cryopreservation of spermatozoa from closed colonies, and inbred, spontaneous mutant, and transgenic strains of rats. |
| ジャーナル | Comp Med |
| Abstract |
We attempted to cryopreserve spermatozoa from closed colonies (Jcl:SD and Jcl:Wistar), and inbred (BN/Crj, F3441 DuCrj, LEW/Crj, Long-Evans and WKY/NCrj), mutant (Zitter [WTC.ZI-zi] and Tremor [TRM]), transgenic (human A-transferase [A], and green fluorescent protein [GFP]) strains of rats. Rat epididymal spermatozoa suspended in cryopreservation solution (23% egg yolk, 8% lactose monohydrate, and 0.7% Equex Stm, pH 7.4, adjusted with 10% Tris [hydroxymethy] aminomethane) were frozen and stored at -196 degrees C. After thawing at 37 degrees C, the spermatozoa were instilled into the tip of each uterine horn of the recipients. A total of five recipient females for each strain were inseminated with cryopreserved spermatozoa, and normal live offspring of all strains (Jcl:SD: 11, Jcl:Wistar: 13, BN/Crj: 9, F344/DuCrj: 28, LEW/Crj: 4, Long-Evans: 6, WKY/NCrj: 8, TRM: 24, WTC.ZI-zi: 27, A: 30 and GFP: 20) were obtained. |
| 巻・号 | 53(6) |
| ページ | 639-41 |
| 公開日 | 2003-12-1 |
| PMID | 14727812 |
| MeSH | Animals Animals, Genetically Modified* Breeding Cryopreservation / methods* Female Fertility / physiology* Fetal Viability / physiology Insemination, Artificial Litter Size Male Pregnancy Pregnancy Outcome Rats Rats, Inbred Strains Rats, Mutant Strains* Semen Preservation Sperm Motility / physiology Spermatozoa* |
| IF | 1.067 |
| 引用数 | 30 |
| WOS 分野 | VETERINARY SCIENCES ZOOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ラット | TRM/Kyo(strainID=11) WTC.ZI-Atrnzi/Kyo(strainID=22) |