| RRC ID |
56001
|
| 著者 |
Todoroki K, Kudoh Y, Nakamura M, Shimizu Y, Sasaki T, Otsuki H, Wada K, Min JZ, Mizuno H, Yoshinari K, Toyo'oka T.
|
| タイトル |
Sensitive and Comprehensive LC-MS/MS Analyses of Chiral Pharmaceuticals and Their Hepatic Metabolites Using Ovomucoid Column.
|
| ジャーナル |
Anal Sci
|
| Abstract |
A sensitive analytical method was developed for the simultaneous detection of 11 chiral pharmaceuticals and their hepatic metabolites by liquid chromatography-tandem mass spectrometry (LC-MS/MS) using an ovomucoid chiral column. After optimization of the LC conditions, all pharmaceuticals examined were enantio-separated with Rs of >0.82 in LC-MS/MS analysis. The limit of detections of all pharmaceuticals by MS/MS detection ranged from 1.2 to 92.3 nM, which is approximately 1000 - 25000 times lower than those obtained by UV detection. From hepatic metabolite analyses in P450-expressing cells, metabolites of three pharmaceuticals were detected and enantio-separated. By using the proposed method, changes in the optical isomer ratio of the hepatic metabolites chlorpheniramine and verapamil caused by differential cytochrome P450 enzyme expression for each isomer, could be successfully traced.
|
| 巻・号 |
34(9)
|
| ページ |
1011-1015
|
| 公開日 |
2018-9-10
|
| DOI |
10.2116/analsci.18SCP05
|
| PMID |
30033954
|
| MeSH |
Chromatography, Liquid / methods*
Hep G2 Cells
Humans
Limit of Detection*
Liver / metabolism*
Pharmaceutical Preparations / chemistry*
Pharmaceutical Preparations / metabolism*
Stereoisomerism
Tandem Mass Spectrometry / methods*
|
| IF |
1.618
|
| 引用数 |
0
|
| リソース情報 |
| ヒト・動物細胞 |
Hep G2 |
