| RRC ID |
56002
|
| Author |
Harimoto N, Nakagawara H, Shirabe K, Yoshizumi T, Itoh S, Ikegami T, Soejima Y, Maehara Y, Ishida Y, Tateno C, Tanaka Y.
|
| Title |
Functional Analysis of Human Hepatocytes Isolated From Chimeric Mouse Liver.
|
| Journal |
Transplant Proc
|
| Abstract |
Chimeric mice with humanized liver were first established by transplanting primary human hepatocytes (PHHs) isolated from a Japanese 27-year-old donor into complementary DNA-urokinase-type plasminogen activator/severe combined immunodeficiency mice. The PHHs from the Japanese donor increased more than 100-fold in the mouse liver, and human hepatocytes purified from the chimeric mouse liver (hcPHs) were successfully transplanted into second-passaged mice. These PHHs and hcPHs can produce human albumin and preserve many liver-specific enzyme genes, which are important for liver function. Interestingly, hepatitis B virus can be infected with these chimeric mice; hepatitis B viral DNA and hepatitis B surface antigen levels were detectable. In conclusion, hcPHs can be an ideal cell source for analysis of human hepatocytes.
|
| Volume |
50(10)
|
| Pages |
3858-3862
|
| Published |
2018-12-1
|
| DOI |
10.1016/j.transproceed.2018.06.035
|
| PII |
S0041-1345(18)30894-7
|
| PMID |
30577278
|
| MeSH |
Animals
Disease Models, Animal*
Hepatocytes / transplantation*
Humans
Mice
Mice, SCID
Transplantation Chimera*
|
| IF |
0.959
|
| Times Cited |
1
|
| Resource |
| Human and Animal Cells |
Hep G2 |
