論文 - 詳細
| RRC ID | 56011 |
|---|---|
| 著者 | Okimura C, Sakumura Y, Shimabukuro K, Iwadate Y. |
| タイトル | Sensing of substratum rigidity and directional migration by fast-crawling cells. |
| ジャーナル | Phys Rev E |
| Abstract |
Living cells sense the mechanical properties of their surrounding environment and respond accordingly. Crawling cells detect the rigidity of their substratum and migrate in certain directions. They can be classified into two categories: slow-moving and fast-moving cell types. Slow-moving cell types, such as fibroblasts, smooth muscle cells, mesenchymal stem cells, etc., move toward rigid areas on the substratum in response to a rigidity gradient. However, there is not much information on rigidity sensing in fast-moving cell types whose size is ∼10 μm and migration velocity is ∼10 μm/min. In this study, we used both isotropic substrata with different rigidities and an anisotropic substratum that is rigid on the x axis but soft on the y axis to demonstrate rigidity sensing by fast-moving Dictyostelium cells and neutrophil-like differentiated HL-60 cells. Dictyostelium cells exerted larger traction forces on a more rigid isotropic substratum. Dictyostelium cells and HL-60 cells migrated in the "soft" direction on the anisotropic substratum, although myosin II-null Dictyostelium cells migrated in random directions, indicating that rigidity sensing of fast-moving cell types differs from that of slow types and is induced by a myosin II-related process. |
| 巻・号 | 97(5-1) |
| ページ | 052401 |
| 公開日 | 2018-5-1 |
| DOI | 10.1103/PhysRevE.97.052401 |
| PMID | 29906928 |
| MeSH | Actomyosin / metabolism Anisotropy Biomechanical Phenomena Cell Differentiation Cell Movement* Dictyostelium / cytology* Dictyostelium / metabolism Elasticity HL-60 Cells Humans Mechanical Phenomena* Myosin Type II / metabolism Time Factors |
| IF | 2.353 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HL60(RCB0041) |