Reference - Detail
| RRC ID | 56027 |
|---|---|
| Author | Schoonenberg VAC, Cole MA, Yao Q, Macias-Treviño C, Sher F, Schupp PG, Canver MC, Maeda T, Pinello L, Bauer DE. |
| Title | CRISPRO: identification of functional protein coding sequences based on genome editing dense mutagenesis. |
| Journal | Genome Biol |
| Abstract |
CRISPR/Cas9 pooled screening permits parallel evaluation of comprehensive guide RNA libraries to systematically perturb protein coding sequences in situ and correlate with functional readouts. For the analysis and visualization of the resulting datasets, we develop CRISPRO, a computational pipeline that maps functional scores associated with guide RNAs to genomes, transcripts, and protein coordinates and structures. No currently available tool has similar functionality. The ensuing genotype-phenotype linear and three-dimensional maps raise hypotheses about structure-function relationships at discrete protein regions. Machine learning based on CRISPRO features improves prediction of guide RNA efficacy. The CRISPRO tool is freely available at gitlab.com/bauerlab/crispro . |
| Volume | 19(1) |
| Pages | 169 |
| Published | 2018-10-19 |
| DOI | 10.1186/s13059-018-1563-5 |
| PII | 10.1186/s13059-018-1563-5 |
| PMID | 30340514 |
| PMC | PMC6195731 |
| MeSH | CRISPR-Cas Systems / genetics* Cell Line Gene Editing* Genome* Humans Molecular Sequence Annotation Mutagenesis / genetics* Open Reading Frames / genetics* Protein Structure, Secondary RNA, Guide, Kinetoplastida / genetics RNA, Messenger / genetics RNA, Messenger / metabolism |
| IF | 14.028 |
| Times Cited | 7 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 42 |
| Altmetric score changes over past 6months | 3.0 |
| Resource | |
| Human and Animal Cells | HUDEP-2(RCB4557) |