論文 - 詳細
| RRC ID | 56070 |
|---|---|
| 著者 | Nagahama M, Takehara M, Miyamoto K, Ishidoh K, Kobayashi K. |
| タイトル | Acid Sphingomyelinase Promotes Cellular Internalization of Clostridium perfringens Iota-Toxin. |
| ジャーナル | Toxins (Basel) |
| Abstract |
Clostridium perfringens iota-toxin is a binary actin-ADP-ribosylating toxin composed of the enzymatic component Ia and receptor binding component Ib. Ib binds to a cell surface receptor, forms Ib oligomer in lipid rafts, and associates with Ia. The Ia-Ib complex then internalizes by endocytosis. Here, we showed that acid sphingomyelinase (ASMase) facilitates the cellular uptake of iota-toxin. Inhibitions of ASMase and lysosomal exocytosis by respective blockers depressed cell rounding induced by iota-toxin. The cytotoxicity of the toxin increased in the presence of Ca2+ in extracellular fluids. Ib entered target cells in the presence but not the absence of Ca2+. Ib induced the extracellular release of ASMase in the presence of Ca2+. ASMase siRNA prevented the cell rounding induced by iota-toxin. Furthermore, treatment of the cells with Ib resulted in the production of ceramide in cytoplasmic vesicles. These observations showed that ASMase promotes the internalization of iota-toxin into target cells. |
| 巻・号 | 10(5) |
| 公開日 | 2018-5-20 |
| DOI | 10.3390/toxins10050209 |
| PII | toxins10050209 |
| PMID | 29783772 |
| PMC | PMC5983265 |
| MeSH | ADP Ribose Transferases / pharmacology* Animals Bacterial Toxins / pharmacology* Biological Transport Dogs Madin Darby Canine Kidney Cells Recombinant Proteins / pharmacology Sphingomyelin Phosphodiesterase / metabolism* |
| IF | 3.895 |
| 引用数 | 2 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MDCK(RCB0995) |