| RRC ID |
56086
|
| 著者 |
Nakano T, Yamamura ET, Fujita H, Sone T, Asano K.
|
| タイトル |
Novel methods for nucleotide length control in double-stranded polyinosinic-polycytidylic acid production using uneven length components.
|
| ジャーナル |
Biosci Biotechnol Biochem
|
| Abstract |
Polyinosinic-polycytidylic acid (PIC), a double-stranded RNA that induces innate immunity in mammals, is a candidate immunopotentiator for pharmaceuticals. The potency and adverse effects of PIC are strongly correlated with the nucleotide length, and the inability to precisely control the length in PIC production limits its practical use. Length extension during the annealing process is the major factor underlying the lack of control, but tuning the annealing conditions is insufficient to resolve this issue. In this study, we developed a novel method to produce accurate nucleotide length PIC at an industrial scale. The length extension was significantly suppressed by the assembly of multiple short polyinosinic acid molecules with one long polycytidylic acid molecule. A newly developed PIC, uPIC100-400, demonstrated a reproducible length and better storage stability than that of corresponding evenly structured PIC. Human dsRNA receptors exhibited equivalent responsiveness to uPIC100-400 and the evenly structured PIC with the same length.
|
| 巻・号 |
82(11)
|
| ページ |
1889-1901
|
| 公開日 |
2018-11-1
|
| DOI |
10.1080/09168451.2018.1501264
|
| PMID |
30079840
|
| MeSH |
Cell Line
Hot Temperature
Humans
Immunity, Innate
Nucleic Acid Conformation*
Nucleotides / chemistry*
Poly C / chemistry
Poly I / chemistry
Poly I-C / chemical synthesis*
Poly I-C / chemistry*
RNA, Double-Stranded / chemistry
|
| IF |
1.297
|
| 引用数 |
2
|
| リソース情報 |
| ヒト・動物細胞 |
MRC-5(RCB0218) |
