Reference - Detail
RRC ID | 56097 |
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Author | Sakagami H, Shi H, Bandow K, Tomomura M, Tomomura A, Horiuchi M, Fujisawa T, Oizumi T. |
Title | Search of Neuroprotective Polyphenols Using the "Overlay" Isolation Method. |
Journal | Molecules |
Abstract |
Previous studies of the neuroprotective activity of polyphenols have used ununiform culture systems, making it difficult to compare their neuroprotective potency. We have established a new and simple method for preparing differentiated PC12 cells by removing the toxic coating step. Cells were induced to differentiate with the nerve growth factor (NGF) in a serum-free medium, without a medium change, but with a one-time overlay supplementation of NGF. The optimal inoculation density of the cells was 6⁻12 × 10³ cells/cm², and the presence of serum inhibited the differentiation. Neuroprotective activity could be quantified by the specific index (SI) value, that is, the ratio of the 50% cytotoxic concentration to the 50% effective concentration. Alkaline extract from the leaves of Sasa senanensis Rehder (SE), having had hormetic growth stimulation, showed the highest SI value, followed by epigallocatechin gallate. The SI value of curcumin and resveratrol was much lower. This simple overly method, that can prepare massive differentiated neuronal cells, may be applicable for the study of the differentiation-associated changes in intracellular metabolites, and the interaction between neuronal cells and physiological factors. |
Volume | 23(8) |
Published | 2018-7-24 |
DOI | 10.3390/molecules23081840 |
PII | molecules23081840 |
PMID | 30042342 |
PMC | PMC6222604 |
MeSH | Amyloid beta-Peptides / antagonists & inhibitors* Amyloid beta-Peptides / toxicity Animals Catechin / analogs & derivatives Catechin / pharmacology Cell Culture Techniques* Cell Differentiation / drug effects Culture Media, Serum-Free / pharmacology Curcumin / pharmacology Hormesis Nerve Growth Factor / pharmacology Neurons / cytology Neurons / drug effects Neurons / metabolism Neuroprotective Agents / isolation & purification Neuroprotective Agents / pharmacology* PC12 Cells Plant Extracts / isolation & purification Plant Extracts / pharmacology* Plant Leaves / chemistry Polyphenols / isolation & purification Polyphenols / pharmacology Rats Resveratrol Sasa / chemistry* Stilbenes / pharmacology Taxoids / antagonists & inhibitors* Taxoids / toxicity |
IF | 3.06 |
Times Cited | 4 |
Resource | |
Human and Animal Cells | PC-12(RCB0009) |