RRC ID 56101
Author Takeuchi M, Doi T, Obayashi K, Hirai A, Yoneda K, Tanaka F, Iwai Y.
Title Soluble PD-L1 with PD-1-binding capacity exists in the plasma of patients with non-small cell lung cancer.
Journal Immunol Lett
Abstract PD-L1 is one of the important immune checkpoint molecules that can be targeted by cancer immunotherapies. PD-L1 has a soluble form (sPD-L1) and a membrane-bound form (mPD-L1). Conventional enzyme-linked immunosorbent assay (ELISA) systems can detect sPD-L1 using anti-PD-L1 capture antibody through the antigen-antibody reaction, but cannot evaluate the quality and function of sPD-L1. In this study, we developed a novel ELISA system for the detection and quantification of sPD-L1 with PD-1-binding capacity (bsPD-L1). To capture bsPD-L1 through the ligand-receptor reaction, the anti-PD-L1 capture antibody in the conventional ELISA was replaced with PD-1-Ig fusion protein in the new ELISA. The new ELISA could detect bsPD-L1 in 29 out of 75 plasma samples from patients with non-small cell lung cancer (NSCLC), with higher sensitivity and frequency than the conventional ELISA. The western blot analysis showed that sPD-L1 in the plasma was glycosylated. Treatment of the samples with glycosidase reduced the absorbance determined by the new ELISA but had no effect on the absorbance determined by the conventional ELISA. These results suggest that glycosylation of sPD-L1 is important for its binding to the immobilized PD-1 in the new ELISA. Our new ELISA system may be useful for the evaluation of functional sPD-L1 with PD-1-binding capacity in cancer patients.
Volume 196
Pages 155-160
Published 2018-4-1
DOI 10.1016/j.imlet.2018.01.007
PII S0165-2478(17)30526-6
PMID 29366663
MeSH Antibodies / immunology B7-H1 Antigen / blood B7-H1 Antigen / immunology* B7-H1 Antigen / metabolism Blotting, Western Carcinoma, Non-Small-Cell Lung / blood Carcinoma, Non-Small-Cell Lung / immunology* Carcinoma, Non-Small-Cell Lung / metabolism Enzyme-Linked Immunosorbent Assay / methods Glycosylation Humans Lung Neoplasms / blood Lung Neoplasms / immunology* Lung Neoplasms / metabolism Programmed Cell Death 1 Receptor / blood Programmed Cell Death 1 Receptor / immunology* Programmed Cell Death 1 Receptor / metabolism Protein Binding Protein Transport Solubility
IF 2.552
Times Cited 17
Human and Animal Cells PC-9(RCB4455)