RRC ID 56105
Author Quan H, Kim Y, Park HC, Yang HC.
Title Effects of phosphatidylserine-containing supported lipid bilayers on the polarization of macrophages.
Journal J Biomed Mater Res A
Abstract Placement of dental implants initiates inflammatory foreign body response, in which macrophages play a central role and affect the subsequent tissue healing process such as bone formation. The purpose of this study was to fabricate phosphatidylserine (PS)-containing supported lipid bilayers (SLBs) on a titanium surface to regulate the polarization of macrophages, a critical factor that affects following tissue healing and regeneration. The fluorescent recovery after photobleaching images showed that the percentage of PS had a critical influence on the fluidity, and 20% PS had the highest fluidity. Furthermore, more expanded and elongated cells were observed in the SLB-coated groups. transforming growth factor-β1 and vascular endothelial growth factor, the key cytokine markers of M2 macrophage polarization, were increased in the SLB-coated groups, especially in the 20% PS group. Consistently, cells cultured on the SLB-coated titanium exhibited the distribution of CD206+ , which is a M2 macrophage specific maker. The results of this study demonstrated M2 polarization of macrophages on PS-SLB-coated titanium discs, which suggests the application of PS-SLB as an immune-regulating coating material to improve tissue reactions to dental implants. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2625-2633, 2018.
Volume 106(10)
Pages 2625-2633
Published 2018-10-1
DOI 10.1002/jbm.a.36454
PMID 29781181
MeSH Animals Cell Adhesion / drug effects Cell Polarity* Cell Proliferation Cell Shape Inflammation Mediators / metabolism Lectins, C-Type / metabolism Lipid Bilayers / chemistry* Macrophages / cytology* Macrophages / ultrastructure Mannose Receptor Mannose-Binding Lectins / metabolism Membrane Fluidity Mice Phosphatidylserines / chemistry* RAW 264.7 Cells RNA, Messenger / genetics RNA, Messenger / metabolism Receptors, Cell Surface / metabolism Titanium / pharmacology Transforming Growth Factor beta1 / genetics Transforming Growth Factor beta1 / metabolism Vascular Endothelial Growth Factor A / metabolism
IF 3.221
Times Cited 3
Human and Animal Cells RAW 264(RCB0535)