論文 - 詳細
| RRC ID | 56107 |
|---|---|
| 著者 | Saiki P, Nakajima Y, Van Griensven LJLD, Miyazaki K. |
| タイトル | Real-time monitoring of IL-6 and IL-10 reporter expression for anti-inflammation activity in live RAW 264.7 cells. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
In previous study, we suggested that the interleukin (IL)-6 and IL-10 could serve as a good biomarker for anti-inflammation that related to chronic inflammatory disease. Recently, we are finding new anti-inflammation compounds from natural products by screening of IL-6 and IL-10 levels. Although, we could measure IL-6 and IL-10 levels by several methods. However, all methods could not measure continuous kinetic of IL-6 and IL-10 levels. Most methods have multiple steps and take a long time. Therefore, there is no a suitable method for screening. To this end, we established IL-6 and IL-10 promoter assay which can monitor with reference gene as Glyceraldehyde 3-phosphate dehydrogenase (gapdh) promoter in living single cell. It could determine IL-6 and IL-10 levels continuously in real-time within two steps. We evaluated IL-6 and IL-10 reporter expression in LPS-induced RAW 264.7 cells with well-known anti-inflammatory compounds such as quercetin, xanthones, β-D-glucan and dexamethasone. As the results, the expression of IL-6 and IL-10 reporters were strongly induced by LPS. The expression of IL-6 reporter was inhibited by all anti-inflammation compounds in LPS-induced RAW 264.7 cells. The expression of IL-10 reporter was inhibited by quercetin, xanthones and dexamethasone in LPS-induced RAW 264.7 cells. While, expression of IL-10 reporter was induced by β-D-glucan. These results indicated that this assay could use for determination of IL-6 and IL-10 reporter expression in LPS-induced RAW 264.7 cells for anti-inflammation activity. Moreover, the results showed that natural compounds have an effect on the time course of IL-6 and IL-10 expressions. Therefore, real-time monitoring has a merit for natural compounds screening. We suggested that this assay could serve as a compound screening assay for anti-inflammation activity. |
| 巻・号 | 505(3) |
| ページ | 885-890 |
| 公開日 | 2018-11-2 |
| DOI | 10.1016/j.bbrc.2018.09.173 |
| PII | S0006-291X(18)32112-0 |
| PMID | 30301531 |
| MeSH | Animals Anti-Inflammatory Agents / pharmacology Dexamethasone / pharmacology Drug Evaluation, Preclinical / methods Drug Monitoring / methods* Inflammation Mediators / analysis* Interleukin-10 / agonists Interleukin-10 / analysis* Interleukin-10 / antagonists & inhibitors Interleukin-6 / agonists Interleukin-6 / analysis* Interleukin-6 / antagonists & inhibitors Lipopolysaccharides / pharmacology Mice Quercetin / pharmacology RAW 264.7 Cells Xanthones / pharmacology beta-Glucans / pharmacology |
| IF | 2.705 |
| 引用数 | 3 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |