RRC ID 56114
Author Kawabata K, Baba N, Sakano T, Hamano Y, Taira S, Tamura A, Baba S, Natsume M, Ishii T, Murakami S, Ohigashi H.
Title Functional properties of anti-inflammatory substances from quercetin-treated Bifidobacterium adolescentis.
Journal Biosci Biotechnol Biochem
Abstract The genus Bifidobacterium is well known to have beneficial health effects. We discovered that quercetin and related polyphenols enhanced the secretion of anti-inflammatory substances by Bifidobacterium adolescentis. This study investigated characteristics of the anti-inflammatory substances secreted by B. adolescentis. The culture supernatant of B. adolescentis with quercetin reduced the levels of inflammatory mediators in activated macrophages. Spontaneous quercetin degradant failed to increase anti-inflammatory activity, while the enhancement of anti-inflammatory activity by quercetin was sustained after washout of quercetin. Physicochemical treatment of the culture supernatant indicated that its bioactive substances may be heat-stable, non-phenolic, and acidic biomolecules with molecular weights less than 3 kDa. Acetate and lactate have little or no effect on nitric oxide production. Taken together, the anti-inflammatory substances secreted by B. adolescentis may be small molecules but not short chain fatty acids. In agreement with these findings, stearic acid was tentatively identified as a bioactive candidate compound.
Volume 82(4)
Pages 689-697
Published 2018-4-1
DOI 10.1080/09168451.2017.1401916
PMID 29165050
MeSH Acetates / metabolism Animals Anti-Inflammatory Agents / chemistry Anti-Inflammatory Agents / isolation & purification Anti-Inflammatory Agents / pharmacology* Bifidobacterium adolescentis / drug effects* Bifidobacterium adolescentis / metabolism Blotting, Western Cell Line Chromatography, Liquid Culture Media Functional Food* Inflammation Mediators / antagonists & inhibitors Inflammation Mediators / metabolism Lactates / metabolism Lipopolysaccharides / pharmacology Mass Spectrometry Mice Molecular Weight Nitric Oxide / antagonists & inhibitors Nitric Oxide / biosynthesis Quercetin / pharmacology* Stearic Acids / pharmacology
IF 1.297
Times Cited 4
Human and Animal Cells RAW 264(RCB0535)