| RRC ID |
56114
|
| 著者 |
Kawabata K, Baba N, Sakano T, Hamano Y, Taira S, Tamura A, Baba S, Natsume M, Ishii T, Murakami S, Ohigashi H.
|
| タイトル |
Functional properties of anti-inflammatory substances from quercetin-treated Bifidobacterium adolescentis.
|
| ジャーナル |
Biosci Biotechnol Biochem
|
| Abstract |
The genus Bifidobacterium is well known to have beneficial health effects. We discovered that quercetin and related polyphenols enhanced the secretion of anti-inflammatory substances by Bifidobacterium adolescentis. This study investigated characteristics of the anti-inflammatory substances secreted by B. adolescentis. The culture supernatant of B. adolescentis with quercetin reduced the levels of inflammatory mediators in activated macrophages. Spontaneous quercetin degradant failed to increase anti-inflammatory activity, while the enhancement of anti-inflammatory activity by quercetin was sustained after washout of quercetin. Physicochemical treatment of the culture supernatant indicated that its bioactive substances may be heat-stable, non-phenolic, and acidic biomolecules with molecular weights less than 3 kDa. Acetate and lactate have little or no effect on nitric oxide production. Taken together, the anti-inflammatory substances secreted by B. adolescentis may be small molecules but not short chain fatty acids. In agreement with these findings, stearic acid was tentatively identified as a bioactive candidate compound.
|
| 巻・号 |
82(4)
|
| ページ |
689-697
|
| 公開日 |
2018-4-1
|
| DOI |
10.1080/09168451.2017.1401916
|
| PMID |
29165050
|
| MeSH |
Acetates / metabolism
Animals
Anti-Inflammatory Agents / chemistry
Anti-Inflammatory Agents / isolation & purification
Anti-Inflammatory Agents / pharmacology*
Bifidobacterium adolescentis / drug effects*
Bifidobacterium adolescentis / metabolism
Blotting, Western
Cell Line
Chromatography, Liquid
Culture Media
Functional Food*
Inflammation Mediators / antagonists & inhibitors
Inflammation Mediators / metabolism
Lactates / metabolism
Lipopolysaccharides / pharmacology
Mass Spectrometry
Mice
Molecular Weight
Nitric Oxide / antagonists & inhibitors
Nitric Oxide / biosynthesis
Quercetin / pharmacology*
Stearic Acids / pharmacology
|
| IF |
1.297
|
| 引用数 |
4
|
| リソース情報 |
| ヒト・動物細胞 |
RAW 264(RCB0535) |
