論文 - 詳細
| RRC ID | 56135 |
|---|---|
| 著者 | Egawa-Takata T, Yoshino K, Hiramatsu K, Nakagawa S, Serada S, Nakajima A, Endo H, Kubota S, Matsuzaki S, Kobayashi E, Ueda Y, Morii E, Inoue M, Naka T, Kimura T. |
| タイトル | Small Cell Carcinomas of the Uterine Cervix and Lung: Proteomics Reveals Similar Protein Expression Profiles. |
| ジャーナル | Int J Gynecol Cancer |
| Abstract |
OBJECTIVE:The phenotypic and pathological features of small cell cervical carcinoma (SMCC) and small small cell lung cancer (SCLC) are very similar; thus, the chemotherapy regimens used for the rare SMCC have been routinely based on regimens used for common SCLC. We set out to explore the protein expression profile similarities between these 2 cancers to prove that linking their therapeutic regimens is justified, with a secondary aim of finding tumor-specific proteins to use as additional biomarkers for more accurate diagnosis of SMCC, and potentially to use as therapeutic targets. METHODS:Protein expression analysis was performed for 3 cases of SMCC and 1 example each of SCLC, mucinous adenocarcinoma of the cervix (MACC), lung mucinous adenocarcinoma (MACL), and squamous cell carcinoma of the cervix (SCC). We used cancer tissue-originated spheroids (CTOS) and isobaric tags for relative and absolute quantitation (iTRAQ)-based comprehensive and quantitative protein expression profile analysis. Expression in corresponding clinical samples was verified by immunohistochemistry. RESULTS:Rather than organ of origin-specific patterns, the SMCC and SCLC samples revealed remarkably similar protein expression profiles-in agreement with their matching tumor pathology phenotypes. Sixteen proteins were expressed at least 2-fold higher in both small cell carcinomas (SMCC and SCLC) than in MACC or SCC. Immunohistochemical analysis confirmed higher expression of creatine kinase B-type in SMCC, compared with MACC and SCC. CONCLUSIONS:We demonstrate a significant overlapping similarity of protein expression profiles of lung and cervical small cell carcinomas despite the significant differences in their organs of origin. |
| 巻・号 | 28(9) |
| ページ | 1751-1757 |
| 公開日 | 2018-11-1 |
| DOI | 10.1097/IGC.0000000000001354 |
| PMID | 30358701 |
| PMC | PMC6221385 |
| MeSH | Carcinoma, Non-Small-Cell Lung / metabolism* Carcinoma, Small Cell / metabolism* Cell Line, Tumor Chromatography, Liquid Female Humans Immunohistochemistry Lung Neoplasms / metabolism* Neoplasm Proteins / biosynthesis* Proteomics / methods Tandem Mass Spectrometry Uterine Cervical Neoplasms / metabolism* |
| IF | 1.746 |
| 引用数 | 2 |
| リソース情報 | |
| ヒト・動物細胞 | TC-YIK(RCB0443) |