論文 - 詳細
| RRC ID | 56142 |
|---|---|
| 著者 | Tani M, Kamata Y, Deushi M, Osaka M, Yoshida M. |
| タイトル | 7-Ketocholesterol enhances leukocyte adhesion to endothelial cells via p38MAPK pathway. |
| ジャーナル | PLoS One |
| Abstract |
7-Ketocholesterol is a major dietary cholesterol oxidation product found in high concentrations in atherosclerotic plaques, which contribute to the development of atherosclerosis. This study aimed to investigate the effects of 7-ketocholesterol on endothelial inflammation, as well as the underlying mechanisms. Pretreatment of human umbilical vein endothelial cells (HUVEC) with 7-ketocholesterol significantly enhanced the total interactions between human monocytic cells (THP-1 cell line) and TNFα-activated HUVECs under physiological flow conditions, compared to pretreatment with cholesterol (TNFα+50 μM cholesterol: 13.1 ± 0.54 cells/CPF, TNFα+50 μM 7-ketocholesterol: 18.9 ± 0.35 cells/CPF, p < 0.01). 7-Ketocholesterol enhanced the expression of E-selectin, ICAM-1, and VCAM-1 proteins. It also activated p38 mitogen-activated protein kinase (MAPK), and treatment with a p38 MAPK inhibitor inhibited both E-selectin expression via ATF-2 activation and 7-ketocholesterol-induced THP-1 adhesion to HUVECs. These findings suggest that 7-ketocholesterol enhances leukocyte-endothelial interactions by upregulating the expression of adhesion molecules, presumably via the p38 MAPK-dependent pathway. |
| 巻・号 | 13(7) |
| ページ | e0200499 |
| 公開日 | 2018-7-31 |
| DOI | 10.1371/journal.pone.0200499 |
| PII | PONE-D-18-10047 |
| PMID | 30063760 |
| PMC | PMC6067699 |
| MeSH | Cell Adhesion* E-Selectin / metabolism Endothelial Cells / cytology* Human Umbilical Vein Endothelial Cells Humans Intercellular Adhesion Molecule-1 / metabolism Ketocholesterols / pharmacology* Leukocytes / cytology* MAP Kinase Signaling System* Monocytes / cytology Oxysterols / chemistry Risk Factors Tumor Necrosis Factor-alpha / metabolism Vascular Cell Adhesion Molecule-1 / metabolism p38 Mitogen-Activated Protein Kinases / metabolism |
| IF | 2.776 |
| 引用数 | 5 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | THP-1 |