Reference - Detail
|Author||Mashiba R, Ishikawa M, Sumiya YU, Kawakatsu K, Tran NK, Nishikata T.|
|Title||Phagocytic Activation of Macrophages with Serum MAF Depends on Engulfment Efficiency and Not Migratory Activity.|
BACKGROUND/AIM:Serum-derived macrophage activating factor (serum MAF) is known to increase the phagocytic activity of macrophages and potentially plays a role in activating cancer immunity. In order to reveal the contributing factors for phagocytic activation, the migratory activity and the efficiency of engulfment was analyzed.
MATERIALS AND METHODS:THP-1 macrophages were induced by 12-O-tetradecanoyl-13-acetate (TPA). The migratory activity and efficiency of engulfment were analyzed by time-lapse imaging and suspension assay, respectively.
RESULTS:While the distance of migration did not change before and after activation with serum MAF, the efficiency of beads internalisation was significantly increased.
CONCLUSION:Phagocytic activation of serum-MAF-treated macrophages was caused by increasing the efficiency of engulfment. This study contributes to the knowledge about the activation of the immune system through phagocytic activation of macrophages.
|MeSH||Cell Line Humans Macrophage Activation / drug effects* Macrophage Activation / immunology* Macrophage Activation / physiology Macrophage-Activating Factors / pharmacology* Macrophages / drug effects* Macrophages / immunology* Phagocytosis / physiology*|
|Human and Animal Cells||THP-1(RCB1189)|