RRC ID 56145
Author Mashiba R, Ishikawa M, Sumiya YU, Kawakatsu K, Tran NK, Nishikata T.
Title Phagocytic Activation of Macrophages with Serum MAF Depends on Engulfment Efficiency and Not Migratory Activity.
Journal Anticancer Res
Abstract BACKGROUND/AIM:Serum-derived macrophage activating factor (serum MAF) is known to increase the phagocytic activity of macrophages and potentially plays a role in activating cancer immunity. In order to reveal the contributing factors for phagocytic activation, the migratory activity and the efficiency of engulfment was analyzed.
MATERIALS AND METHODS:THP-1 macrophages were induced by 12-O-tetradecanoyl-13-acetate (TPA). The migratory activity and efficiency of engulfment were analyzed by time-lapse imaging and suspension assay, respectively.
RESULTS:While the distance of migration did not change before and after activation with serum MAF, the efficiency of beads internalisation was significantly increased.
CONCLUSION:Phagocytic activation of serum-MAF-treated macrophages was caused by increasing the efficiency of engulfment. This study contributes to the knowledge about the activation of the immune system through phagocytic activation of macrophages.
Volume 38(7)
Pages 4295-4298
Published 2018-7-1
DOI 10.21873/anticanres.12727
PII 38/7/4295
PMID 29970564
MeSH Cell Line Humans Macrophage Activation / drug effects* Macrophage Activation / immunology* Macrophage Activation / physiology Macrophage-Activating Factors / pharmacology* Macrophages / drug effects* Macrophages / immunology* Phagocytosis / physiology*
IF 1.935
Times Cited 2
Human and Animal Cells THP-1(RCB1189)