RRC ID 56173
著者 Hu L, Ge A, Wang X, Wang S, Yue X, Wang J, Feng X, Du W, Liu BF.
タイトル Real-time monitoring of immune responses under pathogen invasion and drug interference by integrated microfluidic device coupled with worm-based biosensor.
ジャーナル Biosens Bioelectron
Abstract Immune response to environmental pathogen invasion is a complex process to prevent host from further damage. For quantitatively understanding immune responses and revealing the pathogenic environmental information, real-time monitoring of such a whole dynamic process with single-animal resolution in well-defined environments is highly desired. In this work, an integrated microfluidic device coupled with worm-based biosensor was proposed for in vivo studies of dynamic immune responses and antibiotics interference in infected C. elegans. Individual worms housed in chambers were exposed to the various pathogens and discontinuously manipulated for imaging with limited influence on physiological activities. The expression of immune responses gene (irg-1) was time-lapse measured in intact worms during pathogen infection. Results demonstrated that irg-1 gene could be induced in the presence of P. aeruginosa strain PA14 in a dose-dependent manner, and the survival of infected worm could be rescued under gentamicin or erythromycin treatments. We expect it to be a versatile platform to facilitate future studies on pathogenesis researches and rapid drug screen using C. elegans disease model.
巻・号 110
ページ 233-238
公開日 2018-7-1
DOI 10.1016/j.bios.2018.03.058
PII S0956-5663(18)30243-4
PMID 29625331
MeSH Adaptive Immunity Animals Anti-Bacterial Agents / therapeutic use Biosensing Techniques / instrumentation* Caenorhabditis elegans / genetics Caenorhabditis elegans / immunology* Caenorhabditis elegans / microbiology* Disease Models, Animal Drug Evaluation, Preclinical / instrumentation Equipment Design Humans Lab-On-A-Chip Devices Microfluidic Analytical Techniques / instrumentation* Pseudomonas Infections / drug therapy Pseudomonas Infections / genetics Pseudomonas Infections / immunology Pseudomonas Infections / microbiology* Pseudomonas aeruginosa / drug effects Pseudomonas aeruginosa / immunology*
IF 9.518
引用数 7
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