RRC ID 56188
著者 Lázaro-Peña MI, Díaz-Balzac CA, Bülow HE, Emmons SW.
タイトル Synaptogenesis Is Modulated by Heparan Sulfate in Caenorhabditis elegans.
ジャーナル Genetics
Abstract The nervous system regulates complex behaviors through a network of neurons interconnected by synapses. How specific synaptic connections are genetically determined is still unclear. Male mating is the most complex behavior in Caenorhabditis elegans It is composed of sequential steps that are governed by > 3000 chemical connections. Here, we show that heparan sulfates (HS) play a role in the formation and function of the male neural network. HS, sulfated in position 3 by the HS modification enzyme HST-3.1/HS 3-O-sulfotransferase and attached to the HS proteoglycan glypicans LON-2/glypican and GPN-1/glypican, functions cell-autonomously and nonautonomously for response to hermaphrodite contact during mating. Loss of 3-O sulfation resulted in the presynaptic accumulation of RAB-3, a molecule that localizes to synaptic vesicles, and disrupted the formation of synapses in a component of the mating circuits. We also show that the neural cell adhesion protein NRX-1/neurexin promotes and the neural cell adhesion protein NLG-1/neuroligin inhibits the formation of the same set of synapses in a parallel pathway. Thus, neural cell adhesion proteins and extracellular matrix components act together in the formation of synaptic connections.
巻・号 209(1)
ページ 195-208
公開日 2018-5-1
DOI 10.1534/genetics.118.300837
PII genetics.118.300837
PMID 29559501
PMC PMC5937176
MeSH Animals Axons / metabolism Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism* Cell Adhesion Molecules, Neuronal / metabolism Female Heparitin Sulfate / metabolism* Interneurons / metabolism Male Neurogenesis / genetics* Neurons / metabolism Proteoglycans / metabolism Sensory Receptor Cells / metabolism Synapses / genetics* Synapses / metabolism*
IF 3.564
引用数 3
リソース情報
線虫 tm472 tm734 tm3006