RRC ID 56268
Author Hubka V, Barrs V, Dudová Z, Sklenář F, Kubátová A, Matsuzawa T, Yaguchi T, Horie Y, Nováková A, Frisvad JC, Talbot JJ, Kolařík M.
Title Unravelling species boundaries in the Aspergillus viridinutans complex (section Fumigati): opportunistic human and animal pathogens capable of interspecific hybridization.
Journal Persoonia
Abstract Although Aspergillus fumigatus is the major agent of invasive aspergillosis, an increasing number of infections are caused by its cryptic species, especially A. lentulus and the A. viridinutans species complex (AVSC). Their identification is clinically relevant because of antifungal drug resistance and refractory infections. Species boundaries in the AVSC are unresolved since most species have uniform morphology and produce interspecific hybrids in vitro. Clinical and environmental strains from six continents (n = 110) were characterized by DNA sequencing of four to six loci. Biological compatibilities were tested within and between major phylogenetic clades, and ascospore morphology was characterised. Species delimitation methods based on the multispecies coalescent model (MSC) supported recognition of ten species including one new species. Four species are confirmed opportunistic pathogens; A. udagawae followed by A. felis and A. pseudoviridinutans are known from opportunistic human infections, while A. felis followed by A. udagawae and A. wyomingensis are agents of feline sino-orbital aspergillosis. Recently described human-pathogenic species A. parafelis and A. pseudofelis are synonymized with A. felis and an epitype is designated for A. udagawae. Intraspecific mating assay showed that only a few of the heterothallic species can readily generate sexual morphs in vitro. Interspecific mating assays revealed that five different species combinations were biologically compatible. Hybrid ascospores had atypical surface ornamentation and significantly different dimensions compared to parental species. This suggests that species limits in the AVSC are maintained by both pre- and post-zygotic barriers and these species display a great potential for rapid adaptation and modulation of virulence. This study highlights that a sufficient number of strains representing genetic diversity within a species is essential for meaningful species boundaries delimitation in cryptic species complexes. MSC-based delimitation methods are robust and suitable tools for evaluation of boundaries between these species.
Volume 41
Pages 142-174
Published 2018-12
DOI 10.3767/persoonia.2018.41.08
PMID 30728603
IF 6.86
Resource
Pathogenic eukaryotic microorganisms 病原真菌:IFM 57291T (= CCF 4670T), IFM 57290 (= CCF 4666), IFM 61334T = (JCM 19878T), IFM 61337 (= JCM 19879), IFM 61338 (= JCM 19880), IFM 61339 (= CCF 4903), IFM 61340 = (CCF 4904), IFM 61345 = (CCF 5633), IFM 61346 = (CCF 4906), IFM 61349 (= CCF 4907), IFM 61362 (= CCF 4908), IFM 59922 (= CCF 4560), IFM 59923 (= CCF 4569), IFM 57289 (= CCF 4665), IFM 59502 (= CCF 4561), IFM 59503 (= CCF 4562), IFM 59793T (= KUFC 6349T), IFM 61157 (= KUFC 6397), IFM 46972T (= CBS 114217T), IFM 46973 (= CBS 114218), IFM 5058 (= CCF 4662), IFM 51744 =( CCF 4671), IFM 53868 = (CCF 4667), IFM 54131 = (CCF 4663), IFM 54132 = (CCF 4664), IFM 54745 (= CCF 4661), IFM 55207 (= NBRC 31952), IFM 62155 (= CCF 4668), IFM 59681 (= CCF 4563)