RRC ID 56278
Author Muraosa Y, Toyotome T, Yahiro M, Kamei K.
Title Characterisation of novel-cell-wall LysM-domain proteins LdpA and LdpB from the human pathogenic fungus Aspergillus fumigatus.
Journal Sci Rep
Abstract Aspergillus fumigatus, a filamentous fungus that is ubiquitous in the environment, causes several human pulmonary disorders, including chronic and acute invasive infections and allergic diseases. Lysin motif (LysM) is a small protein domain that binds chitin, a major component of fungal cell wall polysaccharides. Several secreted LysM-domain proteins without catalytic function (LysM effectors) have been identified. They act as virulence factors in plant pathogenic fungi by preventing the immune response induced by chitin; however, LysM proteins in mammalian pathogenic fungi remain largely unexplored. We describe two novel LysM-domain proteins, LdpA and LdpB, in A. fumigatus. Functional analyses of single and double knockouts revealed no significant effects on cell wall chitin content, cell wall integrity, fungal morphology and fungal growth. Fluorescent signals from LdpA-green fluorescent protein (GFP) and LdpB-GFP were observed in cell wall and extracellular matrix. In a mouse model of invasive pulmonary aspergillosis, survival did not differ between ΔldpA/B and wild-type infection; however, further studies are required to reveal their functions in fungal-host interactions.
Volume 9(1)
Pages 3345
Published 2019-3-4
DOI 10.1038/s41598-019-40039-1
PII 10.1038/s41598-019-40039-1
PMID 30833675
PMC PMC6399445
MeSH Animals Aspergillus fumigatus / metabolism* Aspergillus fumigatus / pathogenicity Cell Wall / metabolism* Fungal Proteins / metabolism* Humans Mice
IF 4.011
Times Cited 0
Pathogenic eukaryotic microorganisms IFM 54229 (= Af293)