RRC ID 56284
Author Shigeyasu C, Yamada M, Aoki K, Ishii Y, Tateda K, Yaguchi T, Okajima Y, Hori Y.
Title Metagenomic analysis for detecting Fusarium solani in a case of fungal keratitis.
Journal J Infect Chemother
Abstract PURPOSE:To report a difficult-to-identify case of keratitis due to Fusarium solani, diagnosed with the help of exhaustive gene analysis.
CASE:A 47-year-old woman attended our hospital with a refractory corneal ulcer associated with contact lens wear in her left eye that had appeared two weeks earlier. On her initial visit, slit lamp examination revealed a small double-ringed irregular hyphate ulcer in the center of the cornea, which had no epithelial defect, suggesting fungal infection. Microscopic visualization and culture of corneal scrapings were performed repeatedly, but the results were negative. Despite intensive antifungal treatment, infiltration of the cornea worsened and therapeutic keratoplasty was performed. Specimens from a corneal button were microbiologically and histologically negative for microorganisms. During irrigation of the anterior chamber with amphotericin B, aspirated corneal infiltrations were submitted for culture and metagenomic analysis. Genes belonging to F. solani were identified by metagenomic analysis, and an isolate presumed to be a Fusarium species was cultured. Genotypic identification of the isolates confirmed F. solani.
CONCLUSION:The detection of microorganisms, especially fungi, can be extremely difficult. In difficult-to-culture cases, metagenomic analysis seems to be a promising technique for the identification of microbial pathogens.
Volume 24(8)
Pages 664-668
Published 2018-8-1
DOI 10.1016/j.jiac.2017.12.019
PII S1341-321X(17)30320-3
PMID 29371064
MeSH Amphotericin B / therapeutic use Antifungal Agents / therapeutic use Cornea / microbiology* Cornea / pathology Cornea / surgery Corneal Transplantation DNA, Fungal / genetics DNA, Fungal / isolation & purification Eye Infections, Fungal / diagnosis Eye Infections, Fungal / microbiology* Eye Infections, Fungal / pathology Eye Infections, Fungal / therapy Female Fusarium / genetics* Fusarium / isolation & purification Humans Keratitis / microbiology* Metagenomics* Middle Aged Sequence Analysis, DNA
IF 1.539
Times Cited 3
Pathogenic eukaryotic microorganisms 病原真菌:IFM 63562