RRC ID 56293
著者 Toyotome T, Hamada S, Yamaguchi S, Takahashi H, Kondoh D, Takino M, Kanesaki Y, Kamei K.
タイトル Comparative genome analysis of Aspergillus flavus clinically isolated in Japan.
ジャーナル DNA Res
Abstract Aspergillus flavus is an important zoonotic pathogen and a well-known aflatoxin producer. Aspergillus flavus strains that are prevalent in Japanese environments are reported to be non-aflatoxigenic, although their aflatoxin productivity, especially among clinical isolates, has not been thoroughly investigated to date. In this study, we sequenced the genomes of ten strains of A. flavus isolated in Japan and compared their sequences with each other as well as with those of Aspergillus oryzae RIB40 and A. flavus NRRL3357. The phylogenetic analysis based on identified SNPs indicated that five strains were closer to A. oryzae RIB40 than to A. flavus NRRL3357. In contrast, of those isolates that were closer to A. flavus NRRL3357 than to A. oryzae RIB40, three were found to possess either the entire or partial aflatoxin biosynthesis gene cluster of NRRL3357-type. Furthermore, two of the three actually produced either aflatoxin B1 or an intermediate of the reaction leading to aflatoxin formation. Three of the ten strains we isolated were identified to possess part of the aflatoxin gene cluster, while five others retained the A. oryzae RIB40-type cluster. The genome data thus obtained may be further explored and utilized for comparative analysis of aflatoxin production in environmental and clinical isolates of A. flavus.
巻・号 26(1)
ページ 95-103
公開日 2019-2-1
DOI 10.1093/dnares/dsy041
PII 5231944
PMID 30520983
PMC PMC6379028
MeSH Aflatoxins / biosynthesis* Aspergillus flavus / genetics* Aspergillus flavus / metabolism Aspergillus oryzae / genetics Aspergillus oryzae / metabolism Genomics Japan Metabolic Networks and Pathways / genetics* Multigene Family* Phylogeny Polymorphism, Single Nucleotide Sequence Analysis, DNA
IF 4.0
引用数 2
リソース情報
病原真核微生物 IFM 54693, IFM 55053, IFM 57535, IFM 58503, IFM 59894, IFM 59975, IFM 60519, IFM 60655, IFM 61224, IFM 61226