RRC ID 5631
Author Evans CJ, Olson JM, Ngo KT, Kim E, Lee NE, Kuoy E, Patananan AN, Sitz D, Tran P, Do MT, Yackle K, Cespedes A, Hartenstein V, Call GB, Banerjee U.
Title G-TRACE: rapid Gal4-based cell lineage analysis in Drosophila.
Journal Nat. Methods
Abstract We combined Gal4-UAS and the FLP recombinase-FRT and fluorescent reporters to generate cell clones that provide spatial, temporal and genetic information about the origins of individual cells in Drosophila melanogaster. We named this combination the Gal4 technique for real-time and clonal expression (G-TRACE). The approach should allow for screening and the identification of real-time and lineage-traced expression patterns on a genomic scale.
Volume 6(8)
Pages 603-5
Published 2009-8
DOI 10.1038/nmeth.1356
PII nmeth.1356
PMID 19633663
PMC PMC2754220
MeSH Animals Cell Lineage* Clone Cells DNA Nucleotidyltransferases / genetics* DNA-Binding Proteins / genetics* Drosophila Proteins / genetics* Drosophila melanogaster / cytology Drosophila melanogaster / embryology Drosophila melanogaster / genetics* Fluorometry Genes, Reporter Genetic Techniques* Green Fluorescent Proteins / genetics Open Reading Frames Saccharomyces cerevisiae Proteins / genetics* Transcription Factors / genetics*
IF 26.919
Times Cited 101
WOS Category BIOCHEMICAL RESEARCH METHODS
Resource
Drosophila