RRC ID 56337
Author Fontaine BM, Martin KS, Garcia-Rodriguez JM, Jung C, Briggs L, Southwell JE, Jia X, Weinert EE.
Title RNase I regulates Escherichia coli 2',3'-cyclic nucleotide monophosphate levels and biofilm formation.
Journal Biochem J
Abstract Regulation of nucleotide and nucleoside concentrations is critical for faithful DNA replication, transcription, and translation in all organisms, and has been linked to bacterial biofilm formation. Unusual 2',3'-cyclic nucleotide monophosphates (2',3'-cNMPs) recently were quantified in mammalian systems, and previous reports have linked these nucleotides to cellular stress and damage in eukaryotes, suggesting an intriguing connection with nucleotide/nucleoside pools and/or cyclic nucleotide signaling. This work reports the first quantification of 2',3'-cNMPs in Escherichia coli and demonstrates that 2',3'-cNMP levels in E. coli are generated specifically from RNase I-catalyzed RNA degradation, presumably as part of a previously unidentified nucleotide salvage pathway. Furthermore, RNase I and 2',3'-cNMP levels are demonstrated to play an important role in controlling biofilm formation. This work identifies a physiological role for cytoplasmic RNase I and constitutes the first progress toward elucidating the biological functions of bacterial 2',3'-cNMPs.
Volume 475(8)
Pages 1491-1506
Published 2018-4-30
DOI 10.1042/BCJ20170906
PII BCJ20170906
PMID 29555843
PMC PMC6452634
MeSH Biofilms / growth & development* Escherichia coli / genetics Escherichia coli / growth & development Escherichia coli / metabolism* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Gene Expression Regulation, Bacterial Nucleotides, Cyclic / metabolism* RNA Stability RNA, Bacterial / genetics RNA, Bacterial / metabolism* RNA, Messenger / genetics RNA, Messenger / metabolism* Ribonuclease, Pancreatic / metabolism* Signal Transduction
IF 4.097
Times Cited 7
Prokaryotes E. coli Keio collection