論文 - 詳細
| RRC ID | 56342 |
|---|---|
| 著者 | Kina H, Yoshitani T, Hanyu-Nakamura K, Nakamura A. |
| タイトル | Rapid and efficient generation of GFP-knocked-in Drosophila by the CRISPR-Cas9-mediated genome editing. |
| ジャーナル | Dev Growth Differ |
| Abstract |
The CRISPR-Cas9 technology has been a powerful means to manipulate the genome in a wide range of organisms. A series of GFP knocked-in (GFPKI ) Drosophila strains have been generated through CRISPR-Cas9-induced double strand breaks coupled with homology-directed repairs in the presence of donor plasmids. They visualized specific cell types or intracellular structures in both fixed and live specimen. We provide a rapid and efficient strategy to identify KI lines. This method requires neither co-integration of a selection marker nor prior establishment of sgRNA-expressing transgenic lines. The injection of the mixture of a sgRNA/Cas9 expression plasmid and a donor plasmid into cleavage stage embryos efficiently generated multiple independent KI lines. A PCR-based selection allows to identify KI fly lines at the F1 generation (approximately 4 weeks after injection). These GFPKI strains have been deposited in the Kyoto Drosophila stock center, and made freely available to researchers at non-profit organizations. Thus, they will be useful resources for Drosophila research. |
| 巻・号 | 61(4) |
| ページ | 265-275 |
| 公開日 | 2019-5-1 |
| DOI | 10.1111/dgd.12607 |
| PMID | 31037730 |
| MeSH | Animals CRISPR-Cas Systems / genetics* Drosophila / genetics* Gene Editing / methods* Gene Knock-In Techniques / methods* Green Fluorescent Proteins / genetics* Time Factors |
| IF | 1.638 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#118616 DGRC#118617 DGRC#118618 DGRC#118619 DGRC#118620 DGRC#118621 DGRC#118622 DGRC#118623 DGRC#118624 DGRC#118625 DGRC#118626 DGRC#118651 DGRC#118652 DGRC#118653 |