| RRC ID |
56362
|
| 著者 |
Matsuda S, Okada R, Tandhavanant S, Hiyoshi H, Gotoh K, Iida T, Kodama T.
|
| タイトル |
Export of a Vibrio parahaemolyticus toxin by the Sec and type III secretion machineries in tandem.
|
| ジャーナル |
Nat Microbiol
|
| Abstract |
Many Gram-negative pathogens utilize dedicated secretion systems to export virulence factors such as exotoxins and effectors1-4. Several exotoxins are synthesized as precursors containing amino-terminal Sec signal peptides and are exported through the inner-membrane-bound Sec machinery to the periplasm, followed by secretion across the outer membrane to the exterior using a type II secretion system (T2SS)3,5. Here, we report that thermostable direct haemolysin (TDH), an exotoxin of the food-borne pathogen Vibrio parahaemolyticus, can be exported through the type III secretion system (T3SS), which engages in one-step secretion of effectors4, despite possessing a Sec signal peptide and being mainly secreted via the T2SS. Although the precursor of TDH is targeted to the Sec pathway, a fraction of mature TDH was observed to re-enter the bacterial cytoplasm. The N terminus of mature TDH comprises a T3SS signal sequence, allowing it to be loaded into the T3SS. We also show that T3SS-delivered TDH as an effector contributes to intestinal fluid accumulation in a rabbit diarrhoeal model of V. parahaemolyticus infection. Thus, our results show that an unconventional export mechanism for a bacterial toxin via the T3SS in tandem with the Sec machinery facilitates the virulence trait of V. parahaemolyticus.
|
| 巻・号 |
4(5)
|
| ページ |
781-788
|
| 公開日 |
2019-5-1
|
| DOI |
10.1038/s41564-019-0368-y
|
| PII |
10.1038/s41564-019-0368-y
|
| PMID |
30778145
|
| MeSH |
Animals
Bacterial Proteins / genetics
Bacterial Proteins / metabolism*
Bacterial Toxins / metabolism
Biological Transport
Female
Hemolysin Proteins / metabolism*
Humans
Mice, Inbred C3H
Rabbits
Type III Secretion Systems / genetics
Type III Secretion Systems / metabolism*
Vibrio Infections / microbiology*
Vibrio parahaemolyticus / genetics
Vibrio parahaemolyticus / metabolism*
|
| IF |
14.633
|
| 引用数 |
7
|
| リソース情報 |
| 病原細菌 |
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