RRC ID 564
Author Maeda T, Lee JM, Miyagawa Y, Koga K, Kawaguchi Y, Kusakabe T.
Title Cloning and characterization of a ribonuclease L inhibitor from the silkworm, Bombyx mori.
Journal DNA Seq.
Abstract The ribonuclease L (RNase L) pathway plays an important role in the response of cells to double-stranded RNA (dsRNA) during the events such as virus infection. Ribonuclease L inhibitor (RLI) belonging to the ABC transporter family is known as a regulator of the RNase L pathway. The homologs of RLI were reported in many organisms including the fruit fly and mosquito, but their functions in insects and arthropods have not been elucidated to date. In the present study, we cloned a cDNA of a silkworm RLI homolog, termed BmRLI, and its nucleotide sequence was determined. RT-PCR analysis revealed that the expression of BmRLI mRNA was marked in the testis, ovary and fat body. From the cDNA, recombinant protein with an apparent molecular mass of 69 kDa was expressed in Escherichia coli and cultured insect cells. Although no obvious effect of up-regulation of the BmRLI expression on RNAi was observed, its down-regulation slightly reduced RNAi efficiency.
Volume 16(1)
Pages 21-7
Published 2005-2
DOI 10.1080/10425170400028871
PII XXM7236763P64P74
PMID 16040343
MeSH ATP-Binding Cassette Transporters / genetics* Amino Acid Sequence Animals Base Sequence Bombyx / genetics* Chaperonins / genetics* Cloning, Molecular DNA, Complementary / genetics Endoribonucleases / antagonists & inhibitors* Fat Body / metabolism Female Gene Expression Genes, Insect Insect Proteins / genetics* Male Molecular Sequence Data Ovary / metabolism RNA, Messenger / genetics RNA, Messenger / metabolism Sequence Homology, Amino Acid Testis / metabolism Tissue Distribution
Times Cited 3
Silkworms NA