RRC ID 56459
Author Widyaningrum D, Iida D, Tanabe Y, Hayashi Y, Kurniasih SD, Ohama T.
Title Acutely induced cell mortality in the unicellular green alga Chlamydomonas reinhardtii (Chlorophyceae) following exposure to acrylic resin nanoparticles.
Journal J Phycol
Abstract Nanoparticles have unique properties that make them attractive for use in industrial and medical technology industries but can also be harmful to living organisms, making an understanding of their molecular mechanisms of action essential. We examined the effect of three different sized poly(isobutyl-cyanoacrylate) nanoparticles (iBCA-NPs) on the unicellular green alga Chlamydomonas reinhardtii. We found that exposure to iBCA-NPs immediately caused C. reinhardtii to display abnormal swimming behaviors. Furthermore, after one hour, most of the cells had stopped swimming and 10%-30% of cells were stained with trypan blue, suggesting that these cells had severely impaired plasma membranes. Observation of the cyto-ultrastructure showed that the cell walls had been severely damaged and that many iBCA-NPs were located in the space between the cell wall and plasma membrane, as well as inside the cytosol in some cases. A comparison of three strains of C. reinhardtii with different cell wall conditions further showed that the cell mortality ratio increased more rapidly in the absence of a cell wall. Interestingly, cell mortality over time was essentially identical regardless of iBCA-NP size if the total surface area was the same. Furthermore, direct observation of the trails of iBCA-NPs indicated that the first trigger was their contact with the cell wall, which is most likely accompanied by the inactivation or removal of adsorbed proteins from the cell wall surface. Cell mortality was accompanied by the overproduction of reactive oxygen species, which was detected more readily in cells grown under constant light rather than in the dark.
Volume 55(1)
Pages 118-133
Published 2019-2-1
DOI 10.1111/jpy.12798
PMID 30304548
MeSH Acrylic Resins Chlamydomonas reinhardtii* Chlorophyceae Color Nanoparticles*
IF 2.831
Times Cited 0
Algae NIES-35 NIES-144 NIES-362 NIES-418 NIES-421 NIES-432 NIES-438 NIES-456 NIES-545 NIES-571 NIES-732 NIES-737 NIES-858 NIES-1022 NIES-1048 NIES-1848 NIES-2150 NIES-2169 NIES-2201 NIES-2202 NIES-2208 NIES-2209 NIES-2212 NIES-2280 NIES-2352 NIES-2462 NIES-2578