| RRC ID |
56459
|
| 著者 |
Widyaningrum D, Iida D, Tanabe Y, Hayashi Y, Kurniasih SD, Ohama T.
|
| タイトル |
Acutely induced cell mortality in the unicellular green alga Chlamydomonas reinhardtii (Chlorophyceae) following exposure to acrylic resin nanoparticles.
|
| ジャーナル |
J Phycol
|
| Abstract |
Nanoparticles have unique properties that make them attractive for use in industrial and medical technology industries but can also be harmful to living organisms, making an understanding of their molecular mechanisms of action essential. We examined the effect of three different sized poly(isobutyl-cyanoacrylate) nanoparticles (iBCA-NPs) on the unicellular green alga Chlamydomonas reinhardtii. We found that exposure to iBCA-NPs immediately caused C. reinhardtii to display abnormal swimming behaviors. Furthermore, after one hour, most of the cells had stopped swimming and 10%-30% of cells were stained with trypan blue, suggesting that these cells had severely impaired plasma membranes. Observation of the cyto-ultrastructure showed that the cell walls had been severely damaged and that many iBCA-NPs were located in the space between the cell wall and plasma membrane, as well as inside the cytosol in some cases. A comparison of three strains of C. reinhardtii with different cell wall conditions further showed that the cell mortality ratio increased more rapidly in the absence of a cell wall. Interestingly, cell mortality over time was essentially identical regardless of iBCA-NP size if the total surface area was the same. Furthermore, direct observation of the trails of iBCA-NPs indicated that the first trigger was their contact with the cell wall, which is most likely accompanied by the inactivation or removal of adsorbed proteins from the cell wall surface. Cell mortality was accompanied by the overproduction of reactive oxygen species, which was detected more readily in cells grown under constant light rather than in the dark.
|
| 巻・号 |
55(1)
|
| ページ |
118-133
|
| 公開日 |
2019-2-1
|
| DOI |
10.1111/jpy.12798
|
| PMID |
30304548
|
| MeSH |
Acrylic Resins
Chlamydomonas reinhardtii*
Chlorophyceae
Color
Nanoparticles*
|
| IF |
2.831
|
| 引用数 |
0
|
| リソース情報 |
| 藻類 |
NIES-35
NIES-144
NIES-362
NIES-418
NIES-421
NIES-432
NIES-438
NIES-456
NIES-545
NIES-571
NIES-732
NIES-737
NIES-858
NIES-1022
NIES-1048
NIES-1848
NIES-2150
NIES-2169
NIES-2201
NIES-2202
NIES-2208
NIES-2209
NIES-2212
NIES-2280
NIES-2352
NIES-2462
NIES-2578 |
